Tuning the Biological Activity of RGD Peptides with Halotryptophans†.

Basic Information

ID: ALA4765475

Journal: J Med Chem

Title: Tuning the Biological Activity of RGD Peptides with Halotryptophans†.

Authors: Kemker I,Schröder DC,Feiner RC,Müller KM,Marion A,Sewald N

Abstract: An array of l- and d-halotryptophans with different substituents at the indole moiety was synthesized employing either enzymatic halogenation by halogenases or incorporation of haloindoles using tryptophan synthase. Introduction of these Trp derivatives into RGD peptides as a benchmark system was performed to investigate their influence on bioactivity. Halotryptophan-containing RGD peptides display increased affinity toward integrin αβ and enhanced selectivity over integrin αβ. In addition, bromotryptophan was exploited as a platform for late-stage diversification by Suzuki-Miyaura cross-coupling (SMC), resulting in new-to-nature biaryl motifs. These peptides show enhanced affinity toward αβ, good affinity to αβ, and remarkable selectivity over αβ and αβ while featuring fluorogenic properties. Their feasibility as a probe was demonstrated in vitro. Extensive molecular dynamics simulations were undertaken to elucidate NMR and high-performance liquid chromatography (HPLC) data for these late-stage diversified cyclic RGD peptides and to further characterize their conformational preferences.

CiteXplore: 33356253

DOI: 10.1021/acs.jmedchem.0c01536

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