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ID: ALA4837231

Journal: Eur J Med Chem

Title: Development of peptide epoxyketones as selective immunoproteasome inhibitors.

Authors: Li X, Hong D, Zhang M, Xu L, Zhou Y, Li J, Liu T.

Abstract: A series of epoxyketone analogues with varying N-caps and P3-configurations were designed, synthesized and evaluated. We found that D-Ala in P3 was crucial for β5i selectivity over β5c. Notably, compounds 20j (β5i IC50 = 26.0 nM, 25-fold selectivity) and 20l (β5i IC50 = 25.1 nM, 24-fold selectivity) with the D-configuration at P3 were the most selective inhibitors. Although 20j and 20l showed only moderate anti-proliferative activity against RPMI-8226 and MM.1S cell lines, based on our experiments, it indicates that the inhibition of β5i alone is not sufficient to exert anticancer effects and may rely on the complementary inhibition of β1i, β5c and β5i. These data further increase our understanding of immunoproteasome inhibitors in hematologic malignancies.

CiteXplore: 34087498

DOI: 10.1016/j.ejmech.2021.113556