Document Report Card

ID: ALA5107947

Journal: RSC Med Chem

Title: Estimating the cooperativity of PROTAC-induced ternary complexes using ¹⁹F NMR displacement assay.

Authors: de Castro GV, Ciulli A.

Abstract: Cooperativity is an important parameter to understand the ternary complexes formed by protein degraders. We developed fluorine NMR competition binding experiments to determine cooperativity of PROTACs. We show applicability to estimate both positive and negative cooperativity, also with homo-dimerizers, and highlight key features and considerations for optimal assay development.

CiteXplore: 34778777

DOI: 10.1039/D1MD00215E