Document Report Card
Basic Information
ID: ALA5209585
Title: FFN206 based assay for SLC18A2 using HEK-293 SLC18A2 OE cells
Authors: Sara Tremolada; Loredana Redaelli; Lia Scarabottolo
Abstract: FFN206 (FFN= False Fluorescent Neurotrasmitter) is a fluorescent assay developed in intact cells for monoamine uptake. Compound FFN206 is a fluorescent SLC18A1/SLC18A2 substrate which was developed and allowed functional examination of VMAT1 and VMAT2 in cell culture, using fluorescence microscopy or microplate reader. It crosses the plasma membrane and accumulates into vesicles by SLC18A1 or SLC18A2 transport. SLC18A2 is a transporter protein expressed on vesicular membranes for the monoamines (dopamine, serotonin, norepinephrine, and histamine). It is expressed in the monoaminergic neurons throughout the CNS. It functions as antiporter (2H+ out/1 monoamine in) essential for monoamine uptake into vesicles.