Inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by PAGE analysis
Inhibition of APE1 in human HeLa whole cell extract using [35P]-5'-AP-DNA as substrate assessed as activity remaining at 100 uM incubated for 15 mins prior to substrate addition measured after 5 mins by PAGE analysis relative to control
Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by Lineweaver-Burk plot analysis
Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation incubated for 10 mins prior to substrate addition measured after 5 mins by electrophoretic mobility shift assay
Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation at 30 uM incubated for 10 mins prior to substrate addition measured after 5 mins by electrophoretic mobility shift assay
Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation at 10 to 100 uM preincubated with substrate for 15 mins prior to enzyme addition measured after 5 mins by electrophoretic mobility shift assay
Inhibition of APE1 in human HeLa cells assessed as accumulation of genomic AP sites at 7.5 uM after 24 hrs by aldehyde reactive probe-based assay relative to control
Inhibition of APE1 activity in human HeLa cells assessed as fold increase in genomic AP site accumulation after 24 hrs by aldehyde reactive probe-based assay in presence of methyl methanesulfonate
Potentiation of methyl methanesulfonate cytotoxic activity against human HeLa cells assessed as decrease in cell viability at 5 to 10 uM after 24 hrs by CellTiterGlo assay
Potentiation of temozolomide cytotoxic activity against human HeLa cells assessed as decrease in cell viability at 5 to 10 uM after 24 hrs by CellTiterGlo assay
Potentiation of methyl methanesulfonate cytotoxic activity against human HeLa cells assessed as decrease in cell viability at 1 to 2 uM incubated for 8 hrs measured after 56 hrs post compound wash-out by Hoechst 33342 staining-based assay
Potentiation of temozolomide cytotoxic activity against human HeLa cells assessed as decrease in cell viability at 1 to 2 uM incubated for 8 hrs measured after 56 hrs post compound wash-out by Hoechst 33342 staining-based assay