# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5107637 | B | Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate preincubated for 30 mins followed by substrate addition and measured by malachite green based colorimetric assay relative to control | Escherichia coli | 49 | ALA5104216 | single protein format | Scientific Literature | |
2. | ALA5107638 | B | Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate and measured by malachite green based colorimetric assay relative to control | Escherichia coli | 49 | ALA5104216 | single protein format | Scientific Literature | |
3. | ALA5107639 | B | Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate preincubated for 30 mins in presence of 1 mM DTT followed by substrate addition and measured by malachite green based colorimetric assay relative to control | Escherichia coli | 49 | ALA5104216 | single protein format | Scientific Literature | |
4. | ALA5107641 | B | Inhibition of recombinant SARS-CoV-2 3CLpro expressed in Escherichia coli NiCo21(DE3) assessed as residual activity at 500 uM using DABCYL-KTSAVLQSGFRKME-EDANS as substrate preincubated for 30 mins followed by substrate addition and measured by FRET assay relative to control | Severe acute respiratory syndrome coronavirus 2 | 49 | ALA5104216 | assay format | Scientific Literature | |
5. | ALA5107642 | B | Inhibition of recombinant SARS-CoV-2 3CLpro expressed in Escherichia coli NiCo21(DE3) assessed as residual activity at 500 uM using DABCYL-KTSAVLQSGFRKME-EDANS as substrate preincubated for 30 mins in presence of 1mM DTT followed by substrate addition and measured by FRET assay relative to control | Severe acute respiratory syndrome coronavirus 2 | 49 | ALA5104216 | assay format | Scientific Literature | |
6. | ALA5107643 | B | Inhibition of Mycobacterium tuberculosis MetAP1a assessed as residual activity at 625 uM using L-methionine 7-amido-4-methylcoumarin as fluorogenic substrate and measured by fluorescence based assay | Mycobacterium tuberculosis | 49 | ALA5104216 | assay format | Scientific Literature | |
7. | ALA5107644 | B | Inhibition of recombinant Escherichia coli DdlB assessed as residual activity at 500 uM using D-alanine as substrate incubated for 20 mins and measured by malachite green based colorimetric assay relative to control | Escherichia coli | 49 | ALA5104216 | single protein format | Scientific Literature | |
8. | ALA5107645 | P | Aqueous stability of the compound at pH 7.4 measured after 60 mins by spectrophotometric analysis | 37 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
9. | ALA5107646 | P | Redox activity of compound assessed as redox index at 100 uM measured by HRP-PR assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
10. | ALA5107647 | P | Redox activity of compound assessed as redox index at 100 uM in presence of 1 mM DTT measured by HRP-PR assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
11. | ALA5107648 | P | Redox activity of compound assessed as redox index at 100 uM measured by H2DCFDA assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
12. | ALA5107649 | P | Redox activity of compound assessed as redox index at 100 uM in presence of 100 uM TCEP measured by H2DCFDA assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
13. | ALA5107650 | P | Redox activity of compound assessed as redox index at 10 uM measured by resazurin assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
14. | ALA5107651 | P | Redox activity of compound assessed as redox index at 1 uM measured by resazurin assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
15. | ALA5107652 | P | Thiol reactivity of compound at 100 uM measured by DTNB assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature | ||
16. | ALA5107653 | P | Thiol reactivity of compound measured by TNB2- assay | 49 | ALA5104216 | small-molecule physicochemical format | Scientific Literature |