#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1687390	Binding	Displacement of biotinylated phosphatidylinositol-3,4,5-phosphate from mouse AKT1 PH domain by surface plasmon resonance competitive binding assay	Mus musculus	49	ALA1681784	single protein format	Scientific Literature
2.	ALA1687391	Functional	Cytotoxicity against human BxPC3 cells by MTT assay	Homo sapiens	49	ALA1681784	cell-based format	Scientific Literature
3.	ALA3888333	Binding	SPR Competitive Binding Assay: SPR interaction analyses were performed with a Biacore 2000, using Biacore 2000 Control Software v3.2 and BIAevaluation v4.1 analysis software (Biacore) as described in Mol Cancer Ther 7:2621 (2008). For the competitive binding assays and the Ki determination, PtdIns(3,4,5)phosphate-biotin labeled liposomes (Echelon Biosciences) and SA chips were used with increasing concentrations of the compound tested.		32	ALA3886664	single protein format	BindingDB Database
4.	ALA4482511	Binding	Binding affinity to recombinant human GAB1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	assay format	Patent Bioactivity Data
5.	ALA4482512	Binding	Binding affinity to recombinant human IRS1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	assay format	Patent Bioactivity Data
6.	ALA4482513	Binding	Binding affinity to AKT1 (unknown origin) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	single protein format	Patent Bioactivity Data
7.	ALA4482514	Binding	Inhibition of HGF-stimulated GAB1 Y627 phosphorylation in human T47D cells at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
8.	ALA4482515	Functional	Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 4 days by MTT assay	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
9.	ALA4482516	Functional	Cytotoxicity in human T47D cells assessed as reduction in cell viability incubated for 4 days by MTT assay	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
10.	ALA4482517	Binding	Selectivity index, ratio of Ki for binding affinity to recombinant human IRS1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay Kd for binding affinity to recombinant human GAB1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay	Homo sapiens	2	ALA4482310	assay format	Patent Bioactivity Data
11.	ALA4482518	Binding	Inhibition of HGF-induced IRS1 phosphorylation in human T47D cells assessed as pSer312 IRS1/total-IRS1 ratio level at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins (Rvb = 100 +/- 0.02%)	Homo sapiens	21	ALA4482310	cell-based format	Patent Bioactivity Data
12.	ALA4482519	ADME	Cytotoxicity in human MCF10A cells assessed as reduction in cell viability incubated for 4 days by MTT assay	Homo sapiens	5	ALA4482310	cell-based format	Patent Bioactivity Data