# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1687390 | Binding | Displacement of biotinylated phosphatidylinositol-3,4,5-phosphate from mouse AKT1 PH domain by surface plasmon resonance competitive binding assay | Mus musculus | 49 | ALA1681784 | single protein format | Scientific Literature | |
2. | ALA1687391 | Functional | Cytotoxicity against human BxPC3 cells by MTT assay | Homo sapiens | 49 | ALA1681784 | cell-based format | Scientific Literature | |
3. | ALA3888333 | Binding | SPR Competitive Binding Assay: SPR interaction analyses were performed with a Biacore 2000, using Biacore 2000 Control Software v3.2 and BIAevaluation v4.1 analysis software (Biacore) as described in Mol Cancer Ther 7:2621 (2008). For the competitive binding assays and the Ki determination, PtdIns(3,4,5)phosphate-biotin labeled liposomes (Echelon Biosciences) and SA chips were used with increasing concentrations of the compound tested. | 32 | ALA3886664 | single protein format | BindingDB Database | ||
4. | ALA4482511 | Binding | Binding affinity to recombinant human GAB1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Homo sapiens | 22 | ALA4482310 | assay format | Patent Bioactivity Data | |
5. | ALA4482512 | Binding | Binding affinity to recombinant human IRS1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Homo sapiens | 22 | ALA4482310 | assay format | Patent Bioactivity Data | |
6. | ALA4482513 | Binding | Binding affinity to AKT1 (unknown origin) by surface plasmon resonance assay | Homo sapiens | 22 | ALA4482310 | single protein format | Patent Bioactivity Data | |
7. | ALA4482514 | Binding | Inhibition of HGF-stimulated GAB1 Y627 phosphorylation in human T47D cells at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins | Homo sapiens | 22 | ALA4482310 | cell-based format | Patent Bioactivity Data | |
8. | ALA4482515 | Functional | Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 4 days by MTT assay | Homo sapiens | 22 | ALA4482310 | cell-based format | Patent Bioactivity Data | |
9. | ALA4482516 | Functional | Cytotoxicity in human T47D cells assessed as reduction in cell viability incubated for 4 days by MTT assay | Homo sapiens | 22 | ALA4482310 | cell-based format | Patent Bioactivity Data | |
10. | ALA4482518 | Binding | Inhibition of HGF-induced IRS1 phosphorylation in human T47D cells assessed as pSer312 IRS1/total-IRS1 ratio level at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins (Rvb = 100 +/- 0.02%) | Homo sapiens | 21 | ALA4482310 | cell-based format | Patent Bioactivity Data |