#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1785541	B	Inhibition of His6-tagged recombinant Plasmodium falciparum dihydroorotate dehydrogenase expressed in Escherichia coli using L-dihydroorotate as substrate after 10 to 15 mins by DCIP dye-based assay	Plasmodium falciparum	59	ALA1781999	assay format	Scientific Literature
2.	ALA1785542	B	Inhibition of His6-tagged recombinant Plasmodium berghei dihydroorotate dehydrogenase expressed in Escherichia coli using L-dihydroorotate as substrate after 10 to 15 mins by DCIP dye-based assay	Plasmodium berghei	59	ALA1781999	assay format	Scientific Literature
3.	ALA1785543	B	Inhibition of His6-tagged recombinant human dihydroorotate dehydrogenase expressed in Escherichia coli using L-dihydroorotate as substrate after 10 to 15 mins by DCIP dye-based assay	Homo sapiens	59	ALA1781999	assay format	Scientific Literature
4.	ALA1785544	F	Antimalarial activity against Plasmodium falciparum 3D7 infected in human A positive erythrocytes by [3H]hypoxanthine uptake assay in presence of serum in medium	Plasmodium falciparum	62	ALA1781999	organism-based format	Scientific Literature
5.	ALA1785547	B	Ratio IC50 for His6-tagged recombinant Plasmodium berghei dihydroorotate dehydrogenase to IC50 for His6-tagged recombinant Plasmodium falciparum dihydroorotate dehydrogenase		8	ALA1781999	assay format	Scientific Literature
6.	ALA1785548	P	Partition coefficient, log D of the compound at pH 7.4 by UV-HPLC analysis		21	ALA1781999	small-molecule physicochemical format	Scientific Literature
7.	ALA1785549	P	Aqueous solubility of the compound in phosphate buffer at pH 6.5		21	ALA1781999	small-molecule physicochemical format	Scientific Literature
8.	ALA1785550	A	Plasma protein binding in human by albumin chromatographic method	Homo sapiens	24	ALA1781999	cell-free format	Scientific Literature
9.	ALA1785551	A	Intrinsic clearance in human liver microsome assessed per mg of protein at 1 uM after 60 mins by LC-MS analysis	Homo sapiens	21	ALA1781999	microsome format	Scientific Literature
10.	ALA1785552	A	Intrinsic clearance in mouse liver microsome assessed per mg of protein at 1 uM after 60 mins by LC-MS analysis	Mus musculus	19	ALA1781999	microsome format	Scientific Literature
11.	ALA1785554	A	Cmax in Swiss mouse at 20 mg/kg, po measured after 24 hrs by LC-MS analysis	Mus musculus	8	ALA1781999	organism-based format	Scientific Literature
12.	ALA1785555	A	Time duration above 1 uM plasma concentration in Swiss mouse at 20 mg/kg, po	Mus musculus	8	ALA1781999	organism-based format	Scientific Literature
13.	ALA1785556	T	Toxicity in Swiss mouse assessed as adverse effect at 20 mg/kg, po measured after 24 hrs	Mus musculus	8	ALA1781999	organism-based format	Scientific Literature
14.	ALA3705388	B	Inhibiton Assay: For studying inhibition of Plasmodium or human DHODH enzyme, two assays that are in routine use are described, for example, in Baldwin, et al. (2002) JBiol Chem., 277, 41827-41834, and Baldwin, et al. (2005) J. Biol. Chem., 280. 21847-21853.Briefly, this colorimetric assay monitors the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm (e = 18.8 mM-1cm-1) for measuring DHOD inhibition. The assay was carried out using a solution containing 100 mM HEPES, pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% Triton X-100, 20 micro molar CoQD (coenzyme QD), 200 micro molar L-dihydroorotate, and 120 micro molar DCIP. Reactions are initiated by addition of enzyme to a final concentration in the range of about 5 nM to about 50 nM while maintaining the temperature of a circulating water bath at 25° C. Alternatively, for potent compounds, activity was determined by directly measuring the production of orotic acid at 296 nm (ε = 4.3 mM-1 cm-1).	Plasmodium falciparum	53	ALA3639309	single protein format	BindingDB Database