Inhibition of TPA-induced cPLA2alpha activity in human platelets assessed as inhibition of arachidonic acid production after 60 mins by UV-reverse phase HPLC analysis
Inhibition of rat brain iPLA2 assessed as 10-pyren-1-yldecanoic acid release using 1-palmitoyl-2-(10-pyren-1-yldecanoyl)-sn-glycero-3-phosphocholine as substrate at 10 uM by UV-reverse phase HPLC analysis
Inhibition of pig pancreas group 1B sPLA2 assessed as release of oleic acid using 2-oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (ammonium salt) as substrate at 10 uM by UV-reverse phase HPLC analysis
Drug level in C57Bl6 mouse plasma assessed as 3-(5-carboxypentanoyl)-1-(2-hydroxy-3-(4-octylphenoxy)propyl)-1H-indole-5-carboxylic acid at 100 mg/kg, po after 1 hr by HPLC analysis
Drug level in C57Bl6 mouse plasma assessed as 3-(5-carboxypentanoyl)-1-(2-hydroxy-3-(4-octylphenoxy)propyl)-1H-indole-5-carboxylic acid at 100 mg/kg, po after 2 hrs by HPLC analysis
Drug level in C57Bl6 mouse intestine assessed per gut at 100 mg/kg, po after 2 hrs by HPLC analysis in presence of Helix pomatia beta-glucuronidase HP2
Metabolic stability in CD-1 mouse liver S9 fraction assessed as UGT-mediated glucuronidation of compound by measuring compound remaining at 5 uM preincubated for 3 mins followed by UDPGA addition measured after 60 mins by HPLC/MS analysis
Metabolic stability in CD-1 mouse liver S9 fraction assessed as NADPH CYP450 reductase-mediated compound metabolism by measuring compound remaining in presence of NADPH at 20 uM after 30 mins by HPLC method
Thermodynamic solubility of the compound in phosphate buffer at pH 7.4 at 1 mg sonicated for 10 mins measured after 20 hrs by reverse phase HPLC analysis
Inhibition of human platelet cytosolic phospholipase alpha-2 using 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycerol as substrate after 60 mins by UV-HPLC method
Metabolic stability in rat liver microsomes assessed as NADPH CYP450 reductase-mediated compound metabolism by measuring compound remaining in presence of NADPH at 20 uM after 30 mins by HPLC