# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1825507 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene assessed as HRE remaining activity at 10 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by alkaline phosphatase reporter assay relative to control | Homo sapiens | 47 | ALA1821664 | cell-based format | Scientific Literature | |
2. | ALA1825508 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene at 2.5 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by HRE-luciferase reporter assay relative to control | Homo sapiens | 32 | ALA1821664 | cell-based format | Scientific Literature | |
3. | ALA1825509 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene at 5 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by HRE-luciferase reporter assay relative to control | Homo sapiens | 32 | ALA1821664 | cell-based format | Scientific Literature | |
4. | ALA1825510 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene at 10 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by HRE-luciferase reporter assay relative to control | Homo sapiens | 32 | ALA1821664 | cell-based format | Scientific Literature | |
5. | ALA1825511 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing HRE-AP reporter gene preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by HRE-luciferase reporter assay | Homo sapiens | 8 | ALA1821664 | cell-based format | Scientific Literature | |
6. | ALA1825600 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing VEGF reporter gene assessed as VEGF expression at 10 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by luciferase reporter assay | Homo sapiens | 8 | ALA1821664 | cell-based format | Scientific Literature | |
7. | ALA1825601 | F | Inhibition of HIF1alpha expression in human LN229 cells assessed as reduction of HIF-1alpha accumulation at 20 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by Western blotting | Homo sapiens | 1 | ALA1821664 | cell-based format | Scientific Literature | |
8. | ALA1825603 | F | Inhibition of HIF1alpha expression in human LN229 cells assessed as reduction of HIF-1alpha accumulation at <10 uM preincubated for 1 hr under normoxia condition followed by 24 hrs incubation under hypoxia condition by Western blotting | Homo sapiens | 1 | ALA1821664 | cell-based format | Scientific Literature | |
9. | ALA1825604 | B | Inhibition of hypoxia-induced HIF1 activation in human LN229 cells expressing VEGF reporter gene assessed as VEGF expression at 10 uM after 24 hrs incubation under hypoxia condition by luciferase reporter assay | Homo sapiens | 8 | ALA1821664 | cell-based format | Scientific Literature | |
10. | ALA1942052 | B | Inhibition of HIF1 in human LN229 cells at 10 uM after 1 hr preincubation under hypoxic condition measured after 24 hrs by luciferase reporter gene assay | Homo sapiens | 7 | ALA1938365 | cell-based format | Scientific Literature | |
11. | ALA1942053 | F | Inhibition of hypoxia-induced HIF1alpha accumulation in human LN229 cells at 20 uM after 1 hr preincubation measured after 24 hrs by Western blot analysis | Homo sapiens | 8 | ALA1938365 | cell-based format | Scientific Literature | |
12. | ALA2060139 | B | Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing luciferase, lac Z gene incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by reporter gene assay | Homo sapiens | 15 | ALA2057132 | cell-based format | Scientific Literature | |
13. | ALA2060140 | B | Inhibition of HIF1 transcriptional activity in human LN229 cells expressing VEGF-HRE-V6R and coexpressing lac Z gene at 10 uM incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by luciferase reporter gene assay relative to control | Homo sapiens | 3 | ALA2057132 | cell-based format | Scientific Literature | |
14. | ALA2060141 | F | Downregulation of HIF1alpha protein level in human LN229 cells expressing VEGF-HRE-V6R at 10 uM incubated for 1 hr in normoxia condition followed by 24 hrs in hypoxia condition by Western blot analysis relative to control | Homo sapiens | 4 | ALA2057132 | cell-based format | Scientific Literature | |
15. | ALA2060285 | P | Aqueous solubility of the compound at pH 7.4 | 1 | ALA2057132 | small-molecule physicochemical format | Scientific Literature | ||
16. | ALA2168933 | P | Aqueous solubility of the compound | 1 | ALA2163270 | small-molecule physicochemical format | Scientific Literature | ||
17. | ALA2168934 | B | Inhibition of P300 CH1 domain-mediated HIF1 transcriptional activity in human LN229-V6R cells assessed as reduction in luciferase activity at 2.5 to 10 uM incubated for 1 hr under normoxia followed by 24 hrs under hypoxia by reporter gene assay | Homo sapiens | 1 | ALA2163270 | cell-based format | Scientific Literature | |
18. | ALA2168936 | F | Inhibition of luciferase activity in human LN229-Lux cells at 2.5 to 10 uM incubated for 1 hr under normoxia followed by 24 hrs under hypoxia by reporter gene assay | Homo sapiens | 14 | ALA2163270 | cell-based format | Scientific Literature | |
19. | ALA2169665 | P | 1-Octanol-phosphate buffer distribution coefficient, log P of the compound at pH 7.4 by HPLC analysis | 11 | ALA2163270 | small-molecule physicochemical format | Scientific Literature | ||
20. | ALA2169666 | P | Aqueous solubility of the compound after 30 mins by HPLC analysis | 13 | ALA2163270 | small-molecule physicochemical format | Scientific Literature |