# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1832655 | B | Inhibition of STAT3 in mouse NIH3T3/vSrc nuclear extract assessed as disruption of the Stat3-DNA complex pre-incubated for 30 mins by EMSA analysis | Mus musculus | 16 | single protein format | Scientific Literature | ||
2. | ALA1832656 | B | Inhibition of STAT3 using fluorescent probe 5-carboxyfluorescein-GpYLPQTV-NH2 after 15 mins by fluorescence polarisation assay | 10 | single protein format | Scientific Literature | |||
3. | ALA2025841 | B | Displacement of fluorescein-labeled phosphotyrosine-peptide derived from the interleukin-6 receptor subunit gp130 from the STAT3 after 15 mins by fluorescent polarization assay | 10 | single protein format | Scientific Literature | |||
4. | ALA2025842 | B | Displacement of 5-carboxyfluorescein-labeled GpYLVLDKW from the STAT5b-SH2 domain after 15 mins by fluorescent polarization assay | 10 | single protein format | Scientific Literature | |||
5. | ALA2025843 | B | Displacement of radioligand from the STAT1 after 15 mins by fluorescent polarization assay | 10 | single protein format | Scientific Literature | |||
6. | ALA2025844 | B | Selectivity ratio of Ki for STAT1 to Ki for STAT5b-SH2 domain | 6 | assay format | Scientific Literature | |||
7. | ALA2025845 | B | Selectivity ratio of Ki for STAT3 to Ki for STAT5b-SH2 domain | 6 | assay format | Scientific Literature | |||
8. | ALA2025846 | B | Disruption of preformed STAT5 homodimer in NIH3T3 cells co-expressing human EGFR nuclear extracts by electrophoretic mobility shift assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
9. | ALA2025847 | F | Cytotoxicity against human MV4-11 cells expressing STAT5 after 48 hrs by alamar blue fluorescent staining | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
10. | ALA2025848 | F | Cytotoxicity against human K562 cells expressing STAT5 after 48 hrs by alamar blue fluorescent staining | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
11. | ALA2025849 | F | Induction of apoptosis in human K562 cells assessed as pre-necrotic cells at 40 uM after 24 hrs using annexin-V FITC/PI double staining by flow cytometry (Rvb = 0.36%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
12. | ALA2025850 | F | Induction of apoptosis in human K562 cells assessed as late apoptotic cells at 40 uM after 24 hrs using annexin-V FITC/PI double staining by flow cytometry (Rvb = 5.6%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
13. | ALA2025851 | F | Induction of apoptosis in human K562 cells assessed as viable cells at 40 uM after 24 hrs using annexin-V FITC/PI double staining by flow cytometry (Rvb = 92.8%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
14. | ALA2025852 | F | Induction of apoptosis in human K562 cells assessed as early apoptotic cells at 40 uM after 24 hrs using annexin-V FITC/PI double staining by flow cytometry (Rvb = 1.26%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
15. | ALA2025853 | A | Cytotoxicity against human Bone marrow cells up to 160 uM after 72 hrs by alamar blue fluorescent staining | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
16. | ALA2025854 | B | Inhibition of BCR-ABL-mediated STAT5 phosphorylation in human K562 cells after 6 hrs by Western blotting analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
17. | ALA2025855 | B | Inhibition of BCR-ABL-mediated STAT5 phosphorylation in human K562 cells after 6 hrs by phospho-flow cytometry | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
18. | ALA2025856 | B | Inhibition of BCR-ABL-mediated STAT3 phosphorylation in human K562 cells at 40 uM after 6 hrs by phospho-flow cytometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
19. | ALA2025857 | B | Inhibition of BCR-ABL-mediated AKT phosphorylation in human K562 cells at 40 uM after 6 hrs by phospho-flow cytometry | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
20. | ALA2025858 | B | Inhibition of BCR-ABL-mediated ERK1 phosphorylation in human K562 cells at 40 uM after 6 hrs by phospho-flow cytometry | Homo sapiens | 2 | cell-based format | Scientific Literature |