# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1919133 | B | Antagonist activity at Escherichia coli recombinant C-terminal 6His-tagged FimH-CRD with a C-terminal thrombin cleavage site expressed in Escherichia coli HM125 assessed as inhibition of streptavidin-horseradish peroxidase conjugated TM-PAA polymer binding after 3 hrs by spectrophotometry | Escherichia coli | 31 | assay format | Scientific Literature | ||
2. | ALA1919134 | B | Ratio of compound IC50 to n-heptyl alpha-D-mannopyranoside IC50 for Escherichia coli recombinant C-terminal 6His-tagged FimH-CRD with a C-terminal thrombin cleavage site expressed in Escherichia coli HM125 assessed as inhibition of streptavidin-horseradish peroxidase conjugated TM-PAA polymer binding after 3 hrs by spectrophotometry | Escherichia coli | 30 | assay format | Scientific Literature | ||
3. | ALA1919137 | P | Octanol-phosphate buffer distribution coefficient, log D of the compound at pH 7.4 after 2 hrs by shake flask method using LC-MS analysis | 31 | small-molecule physicochemical format | Scientific Literature | |||
4. | ALA1919138 | A | Permeability of the compound at pH 5 to 7.4 after 16 hrs by PAMPA assay | 31 | assay format | Scientific Literature | |||
5. | ALA1919193 | P | Thermodynamic solubility of the compound at pH 6.5 after 24 hrs by shake flask method using LC-MS analysis | 31 | small-molecule physicochemical format | Scientific Literature | |||
6. | ALA1919194 | A | Permeability of the compound assessed as membrane retention at pH 5 to 7.4 after 16 hrs by PAMPA assay | 31 | assay format | Scientific Literature | |||
7. | ALA3861501 | B | Inhibition of Escherichia coli UTI89 FimH assessed as decrease in hemagglutination of guinea pig red blood cells after 4 to 18 hrs | Escherichia coli UTI89 | 24 | assay format | Scientific Literature | ||
8. | ALA4820002 | B | Antagonist activity at recombinant 6His-tagged thrombin cleavage site of FimH Lectin domain expressed in Escherichia coli HM125 assessed as dissociation constant incubated for 24 hrs in presence of FITC-labeled tracer by competitive fluorescence polarization assay | Escherichia coli | 26 | assay format | Scientific Literature | ||
9. | ALA4820003 | B | Antagonist activity at recombinant 6His-tagged thrombin cleavage site of FimH Lectin domain R98A mutant expressed in Escherichia coli HM125 assessed as dissociation constant measured after 24 hrs by fluorescence polarization assay | Escherichia coli | 26 | assay format | Scientific Literature |