# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1948717 | Binding | Inhibition of dihydroorotate dehydrogenase in Wistar rat liver mitochondrial/microsomal membranes measured for 5 mins by 2,6-dichlorophenolindophenol reduction-based spectrophotometry | Rattus norvegicus | 14 | ALA1944517 | cell membrane format | Scientific Literature | |
2. | ALA4006962 | Binding | Inhibition of recombinant human N-terminal truncated GST-tagged DHFR (31 to 395 residues) expressed in Escherichia coli BL21(DE3) pyrD using DHO as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by DCIP oxidation based CoQ10 enzyme coupled assay | Homo sapiens | 13 | ALA4004863 | assay format | Scientific Literature | |
3. | ALA4006963 | Functional | Antiproliferative activity against human Jurkat T cells assessed as DNA content after 72 hrs by Hoechst 33258 dye based fluorometric method | Homo sapiens | 13 | ALA4004863 | cell-based format | Scientific Literature | |
4. | ALA4006964 | ADME | Cytotoxicity against human Jurkat T cells assessed as compound concentration required to induce up to 30% cell death after 72 hrs by Hoechst 33258 dye based fluorometric method | Homo sapiens | 13 | ALA4004863 | cell-based format | Scientific Literature | |
5. | ALA4006968 | Functional | Immunosuppressive activity against human PBMC assessed as inhibition of PHA-stimulated cell proliferation preincubated for 2 hrs followed by PHA stimulation for 72 hrs by BrdU incorporation assay | Homo sapiens | 13 | ALA4004863 | cell-based format | Scientific Literature |