#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1961096	B	Inhibition of Plasmodium falciparum C-terminal hexa-His tagged DHODH expressed in Escherichia coli by DCIP reduction assay	Plasmodium falciparum	83		assay format	Scientific Literature
2.	ALA1961097	B	Inhibition of Plasmodium berghei C-terminal hexa-His tagged DHODH expressed in Escherichia coli by DCIP reduction assay	Plasmodium berghei	83		assay format	Scientific Literature
3.	ALA1961098	B	Inhibition of human C-terminal hexa-His tagged DHODH expressed in Escherichia coli by DCIP reduction assay	Homo sapiens	83		assay format	Scientific Literature
4.	ALA1961099	F	Antimalarial activity against Plasmodium falciparum 3D7 by [3H]hypoxanthine incorporation assay in presence of human serum	Plasmodium falciparum	83		organism-based format	Scientific Literature
5.	ALA1961102	P	Lipophilicity, log D of the compound at pH 7.4		25		small-molecule physicochemical format	Scientific Literature
6.	ALA1961103	P	Aqueous solubility of the compound at pH 6.5		25		small-molecule physicochemical format	Scientific Literature
7.	ALA1960900	A	Plasma protein binding in human by chromatographic method	Homo sapiens	25		cell-free format	Scientific Literature
8.	ALA1960902	A	Intrinsic clearance in human liver microsomes	Homo sapiens	24		microsome format	Scientific Literature
9.	ALA1960903	A	Intrinsic clearance in mouse liver microsomes	Mus musculus	25		microsome format	Scientific Literature
10.	ALA3296344	B	Inhibition of mouse DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	Mus musculus	8		cell-based format	Scientific Literature
11.	ALA3296345	F	Antimalarial activity against Plasmodium falciparum 3D7 infected in RBC after 72 hrs by SYBR green dye based flow cytometry	Plasmodium falciparum	7		organism-based format	Scientific Literature
12.	ALA3296346	B	Inhibition of N-terminal His6-tagged recombinant Plasmodium falciparum DHODH (amino acid residues 158 to 569) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	Plasmodium falciparum	7		cell-based format	Scientific Literature
13.	ALA3296347	B	Inhibition of C-terminal His6-tagged human DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	Homo sapiens	8		cell-based format	Scientific Literature
14.	ALA3296348	B	Inhibition of rat DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	Rattus norvegicus	8		cell-based format	Scientific Literature
15.	ALA3296349	B	Inhibition of dog DHODH (amino acid residues 48 to 414) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	Canis lupus familiaris	8		cell-based format	Scientific Literature
16.	ALA3705388	B	Inhibiton Assay: For studying inhibition of Plasmodium or human DHODH enzyme, two assays that are in routine use are described, for example, in Baldwin, et al. (2002) JBiol Chem., 277, 41827-41834, and Baldwin, et al. (2005) J. Biol. Chem., 280. 21847-21853.Briefly, this colorimetric assay monitors the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm (e = 18.8 mM-1cm-1) for measuring DHOD inhibition. The assay was carried out using a solution containing 100 mM HEPES, pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% Triton X-100, 20 micro molar CoQD (coenzyme QD), 200 micro molar L-dihydroorotate, and 120 micro molar DCIP. Reactions are initiated by addition of enzyme to a final concentration in the range of about 5 nM to about 50 nM while maintaining the temperature of a circulating water bath at 25° C. Alternatively, for potent compounds, activity was determined by directly measuring the production of orotic acid at 296 nm (ε = 4.3 mM-1 cm-1).	Plasmodium falciparum	53		single protein format	BindingDB Database
17.	ALA3878218	B	Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli using L-dihydroorotate as substrate by DCIP-based spectrophotometric method	Plasmodium falciparum	7		assay format	Scientific Literature
18.	ALA5246246	F	Antimalarial activity against Chloroquine resistant Plasmodium falciparum W2	Plasmodium falciparum	4		organism-based format	Scientific Literature