#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2025951	Binding	Displacement of [3H] epibatidine from rat alpha2beta2 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
2.	ALA2025952	Binding	Displacement of [3H] epibatidine from rat alpha2beta4 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
3.	ALA2025953	Binding	Displacement of [3H] epibatidine from rat alpha3beta2 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
4.	ALA2025954	Binding	Displacement of [3H] epibatidine from rat alpha3beta4 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
5.	ALA2025955	Binding	Displacement of [3H] epibatidine from rat alpha4beta2 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
6.	ALA2025956	Binding	Displacement of [3H] epibatidine from rat alpha4beta2* nAChR in rat forebrain by beta scintillation counting	Rattus norvegicus	10	ALA2021777	assay format	Scientific Literature
7.	ALA2025957	Binding	Displacement of [3H] epibatidine from rat alpha4beta4 nAChR by beta scintillation counting	Rattus norvegicus	10	ALA2021777	protein complex format	Scientific Literature
8.	ALA2025958	Binding	Selectivity ratio of Ki for rat alpha3beta4 to Ki for rat alpha4beta2	Rattus norvegicus	11	ALA2021777	assay format	Scientific Literature
9.	ALA2025963	Functional	Agonist activity at human alpha4beta2 nAChR expressed in SH-EP1 cells assessed as 86Rb+ ion efflux after 9.5 mins by flip-plate technique	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
10.	ALA2025964	Functional	Agonist activity at human high sensitive and low sensitive alpha4beta2 nAChR expressed in SH-EP1 cells assessed as 86Rb+ ion efflux by flip-plate technique relative to sazetidine-A	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
11.	ALA2025965	Functional	Partial agonist activity at human high sensitive alpha4beta2 nAChR expressed in SH-EP1 cells assessed as 86Rb+ ion efflux by flip-plate technique relative to sazetidine-A	Homo sapiens	10	ALA2021777	cell-based format	Scientific Literature
12.	ALA2025966	Functional	Antagonist activity at human alpha4beta2 nAChR expressed in SH-EP1 cells assessed as inhibition of carbamylcholine induced 86Rb+ ion efflux preincubated for 10 mins prior to carbamylcholine-induction measured after 5 mins by flip-plate technique	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
13.	ALA2025967	Functional	Antagonist activity at human high sensitive and low sensitive alpha4beta2 nAChR expressed in SH-EP1 cells assessed as inhibition of carbamylcholine induced 86Rb+ ion efflux preincubated for 10 mins prior to carbamylcholine-induction measured after 5 mins by flip-plate technique relative to control	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
14.	ALA2025968	Functional	Agonist activity at human alpha1beta1gammadelta nAChR expressed in TE671/RD cells at 10 uM by 86Rb+ flux assay	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
15.	ALA2025969	Functional	Antagonist activity at human alpha1beta1gammadelta nAChR expressed in TE671/RD cells at 10 uM by 86Rb+ flux assay	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
16.	ALA2025970	Functional	Agonist activity at alpha3beta4alpha5beta2 in human SH-SY5Y cells at 10 uM by 86Rb+ flux assay	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
17.	ALA2025971	Functional	Antagonist activity at alpha3beta4alpha5beta2 in human SH-SY5Y cells at 10 uM by 86Rb+ flux assay	Homo sapiens	11	ALA2021777	cell-based format	Scientific Literature
18.	ALA2025972	Functional	Antidepressant activity in BALB/cJ mouse assessed as decrease in immobility at 10 mg/kg, po administered 30 mins prior to testing measured for 6 mins by forced swim test	Mus musculus	1	ALA2021777	organism-based format	Scientific Literature
19.	ALA2025974	Functional	Ex-vivo beta2* nAChR receptor occupancy in mouse brain at 10 mg/kg, ip administered 30 mins prior to testing	Mus musculus	3	ALA2021777	organism-based format	Scientific Literature
20.	ALA2025975	Binding	Displacement of [3H]8-OH-DPAT from 5-HT1A at 10 uM		3	ALA2021777	single protein format	Scientific Literature