#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2028071	F	MMV: Inhibition of Plasmodium falciparum 3D7 (EC50).	Plasmodium falciparum	317		organism-based format	MMV Malaria Box
2.	ALA2028073	F	MMV: Inhibition of Trypanosoma cruzi (Tulahuen C4 LacZ) (Chagas in vitro).	Trypanosoma cruzi	400		organism-based format	MMV Malaria Box
3.	ALA2028074	F	MMV: Inhibition of Trypanosoma brucei brucei (Squib 427) (HAT in vitro).	Trypanosoma brucei brucei	400		organism-based format	MMV Malaria Box
4.	ALA2028075	F	MMV: Inhibition of Trypanosoma brucei rhodesiense (STIB 900) (HAT in vitro).	Trypanosoma brucei rhodesiense	400		organism-based format	MMV Malaria Box
5.	ALA2028076	F	MMV: Inhibition of Leishmania infantum (MHOM/MA/BE/67) in vitro.	Leishmania infantum	400		organism-based format	MMV Malaria Box
6.	ALA2028077	T	MMV: Cytotoxicity against human fibroblasts (MRC-5) cells.	Homo sapiens	400		cell-based format	MMV Malaria Box
7.	ALA2094261	F	MMV: Screen against Mtb, single point, non replicating, at 25uM	Mycobacterium tuberculosis	399		organism-based format	MMV Malaria Box
8.	ALA2094262	F	MMV: Screen against Mtb, single point, replicating, at 25uM	Mycobacterium tuberculosis	399		organism-based format	MMV Malaria Box
9.	ALA2363023	F	MMV: Toxicity @ 2.5 uM to newly transformed Schistosoma mansoni somules after 24 h on a scale of 0 (none) - 4 (most) as judged visually: includes short descriptors of effects (PMID:19597541). Caffrey group UCSF.	Schistosoma mansoni	400		organism-based format	MMV Malaria Box
10.	ALA2363024	F	MMV: Toxicity @ 2.5 uM to newly transformed Schistosoma mansoni somules after 48 h on a scale of 0 (none) - 4 (most) as judged visually: includes short descriptors of effects (PMID:19597541). Caffrey group UCSF.	Schistosoma mansoni	400		organism-based format	MMV Malaria Box
11.	ALA2363025	F	MMV: Toxicity @ 2.5 uM to newly transformed Schistosoma mansoni somules after 72 h on a scale of 0 (none) - 4 (most) as judged visually: includes short descriptors of effects (PMID:19597541). Caffrey group UCSF.	Schistosoma mansoni	400		organism-based format	MMV Malaria Box
12.	ALA2448810	F	MMV: Compounds (1uM) were screened in a 72hr growth assay monitored by flow cytometry both in the presence and absence of supplemental isopentenyl pyrophosphate (IPP) 200uM. Compounds which fail to show anti-malarial activity when chemically rescued by IPP are therefore specific to the apicoplast. The values are expressed as the ratio of the corresponding activity with and without IPP, respectively.	Plasmodium falciparum	400		organism-based format	MMV Malaria Box
13.	ALA3137357	F	MMV: Cryptosporidium parvum oocysts (Iowa Strain) were prepared for use by treatment with 10 mM HCl (37 degrees C, 10 min) followed by 2 mM sodium taurocholate in phosphate-buffered saline (PBS) with Ca2+ and Mg2+ (16 degrees C, 10 min) in order to stimulate excystation. The high-throughput screen was carried out by inoculating >90% confluent human ileocecal adenocarcinoma (HCT-8) cells (ATCC) with ~5.5 x 10^3 primed oocysts per well. Experimental compounds or DMSO (vehicle) were added three hours after infection, and cells were incubated for 48 hours.	Cryptosporidium parvum	400		organism-based format	MMV Malaria Box
14.	ALA3137362	F	MMV: Growth inhibition of P. falciparum cultures was quantified using a parasite lactate dehydrogenase (LDH) assay. The cultures were diluted to 0.5% parasitemea and 2% hematocrit and subjected to each twofold serial dilutions of the compounds for 72 h (DMSO final concentration, 0.25%). After 72 h, the cultures were frozen. LDH activity of P. falciparum parasites in aliquots of freeze-thawed cultures was spectrophotometrically measured and the IC50s were determined.	Plasmodium falciparum	40		organism-based format	MMV Malaria Box
15.	ALA3137382	F	MMV: Percent motility assay at a compound concentration of 1.25e-5 M. Activity score legend: 3: Active, 2: Moderately active; 1: Inactive, 0: Toxic	Onchocerca lienalis	399		organism-based format	MMV Malaria Box
16.	ALA3137383	F	MMV: Percent motility assay at a compound concentration of 3.1e-6 M. Activity score legend: 3: Active, 2: Moderately active; 1: Inactive, 0: Toxic	Onchocerca lienalis	399		organism-based format	MMV Malaria Box
17.	ALA3137384	F	MMV: Percent motility assay at a compound concentration of 1.9e-7 M. Activity score legend: 3: Active, 2: Moderately active; 1: Inactive, 0: Toxic	Onchocerca lienalis	399		organism-based format	MMV Malaria Box
18.	ALA3137385	F	MMV: Percent motility assay at a compound concentration of 7.8e-7 M. Activity score legend: 3: Active, 2: Moderately active; 1: Inactive, 0: Toxic	Onchocerca lienalis	399		organism-based format	MMV Malaria Box
19.	ALA3301449	F	MMV: Primary screening was performed with each compound at a 30 uM final concentration and in triplicate in sealed 96-well polystyrene microtiter plates. Parasite inocula (100 ul) comprising 20000 parasites/ml were added to each well and grown at 37 degrees C in a humidified atmosphere of 5% CO2 for 72 h. Positive controls consisted of metronidazole at 1 mg/ml. The growth of E. histolytica trophozoites in each well was determined microscopically by measuring the diameters of the confluent cells in drug-containing wells relative to those in the nega- tive-control wells.	Entamoeba histolytica	400		organism-based format	MMV Malaria Box
20.	ALA3301450	F	MMV: A total of 100ul of inoculum was added to each well (parasite/cell ratio, ~10:1; final volume, 200 ul). Six hours after inoculation, nonadherent parasites were removed, and 100 ul of complete DMEM (1% penicillin-streptomycin, 3% FBS) supplemented with inhibitors at different concentrations (2-fold serial dilutions starting from 30 uM) was added to all except the negative-control wells. Positive controls, consisting of pyrimethamine (PYR) and sulfadiazine (SDZ) (20-mg/ml stocks in DMSO), were tested at a 2mg/ml final concentration. Each test was performed in triplicate.	Toxoplasma gondii	400		organism-based format	MMV Malaria Box