#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2033953	B	Inhibition in GST-tagged Bcl2 using [FAM]-IWIAQELRRIGDEFNAYY-NH2 as substrate after 60 mins by fluorescence polarization assay		23		single protein format	Scientific Literature
2.	ALA2033954	B	Inhibition of Bcl-XL using fluoresceinated 18-mer Bim as substrate after 60 mins by FRET analysis		23		single protein format	Scientific Literature
3.	ALA2033955	F	Induction of apoptosis in human SU-DHL4 cells assessed as cytochrome c release at 10 uM after 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
4.	ALA2033956	F	Induction of apoptosis in human SU-DHL4 cells assessed as BAK oligomerization at 10 uM after 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
5.	ALA2034351	F	Antiproliferative activity against human SU-DHL4 cells after 72 hrs by Cell-Titer Glo assay in presence of 2% FBS	Homo sapiens	7		cell-based format	Scientific Literature
6.	ALA2034352	F	Antiproliferative activity against human MV411 cells after 72 hrs by Cell-Titer Glo assay in presence of 2% FBS	Homo sapiens	7		cell-based format	Scientific Literature
7.	ALA2034353	F	Antiproliferative activity against human SU-DHL4 cells after 72 hrs by Cell-Titer Glo assay in presence of 10% FBS	Homo sapiens	7		cell-based format	Scientific Literature
8.	ALA2034354	F	Antiproliferative activity against human MV411 cells after 72 hrs by Cell-Titer Glo assay in presence of 10% FBS	Homo sapiens	7		cell-based format	Scientific Literature
9.	ALA3888503	B	Fluorescence Polarization Assay (FPA): The assays were performed in black flat-bottom 384-well plates. The final assay volume was 50 μl prepared from additions of Gst-Bcl-2 (Bcl-2: GENBANK® Accession No. P10415), fluoresceinated 18-mer BIM peptide (NH2-YYANFEDGIRRLEQAIWI-[FAM]) (SEQ ID NO: 1), and test compounds in assay buffer consisting of 20 mM Sodium Phosphate, 1 mM EDTA, 50 mM NaCl, and 0.05% Pluronic Acid. The reaction was incubated at room temperature for 60 minutes and fluorescence polarization of the reaction was detected on the LJL Plate Reader. Inhibition data were calculated from mP values generated by the no protein control reactions for 100% inhibition and vehicle-only reactions for 0% inhibition. The final concentrations of reagents in the assay were 4.9 nM BCL-2, 5 nM fluoresceinated 18-mer BIM peptide and 1% DMSO.	Homo sapiens	93		single protein format	BindingDB Database
10.	ALA3888504	B	Fluorescence Resonance Energy Transfer Assay (FRET): The assays were performed in black flat-bottom 384-well plates. The final assay volume was 55 μl prepared from additions of Biotin-Bcl-xL (Bcl-xL: GENBANK® Accession No. Q07817), fluoresceinated 18-mer BIM peptide (NH2-YYANFEDGIRRLEQAIWI-[FAM]) (SEQ ID NO: 1), Streptavidin-Terbium FRET detection reagent, and test compounds in assay buffer consisting of 20 mM Sodium Phosphate, 1 mM EDTA, 50 mM NaCl, and 0.05% Pluronic Acid. The reaction was incubated at room temperature for 60 minutes. After 60 minutes, 5 μL of Streptavidin-Terbium FRET detection reagent (Perkin Elmer) was added to the reaction mixture and incubated at room temperature in the dark for 30 mins. The FRET signal generated by the reaction was detected on the Envision Plate Reader Inhibition data were calculated from FRET values generated by the no protein control reactions for 100% inhibition and vehicle-only reactions for 0% inhibition. The final concentration of reagents in the assay was 10 nM Bcl-xL, 5 nM fluo	Homo sapiens	93		single protein format	BindingDB Database