# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2045017 | B | Inhibition of human topoisomerase 2alpha-mediated relaxation of supercoiled pBR322 plasmid DNA after 15 mins using ethidium bromide staining by agarose-gel electrophoresis | Homo sapiens | 2 | ALA2040718 | single protein format | Scientific Literature | |
2. | ALA2045018 | B | Complete inhibition of human topoisomerase 2alpha-mediated relaxation of supercoiled pBR322 plasmid DNA at 100 uM after 15 mins using ethidium bromide staining by agarose-gel electrophoresis | Homo sapiens | 4 | ALA2040718 | single protein format | Scientific Literature | |
3. | ALA2045019 | B | Complete inhibition of human topoisomerase 2alpha-mediated relaxation of supercoiled pBR322 plasmid DNA at 50 uM after 15 mins using ethidium bromide staining by agarose-gel electrophoresis | Homo sapiens | 4 | ALA2040718 | single protein format | Scientific Literature | |
4. | ALA2045020 | B | Partial inhibition of human topoisomerase 2alpha-mediated relaxation of supercoiled pBR322 plasmid DNA at 25 uM after 15 mins using ethidium bromide staining by agarose-gel electrophoresis | Homo sapiens | 4 | ALA2040718 | single protein format | Scientific Literature | |
5. | ALA3635921 | B | Inhibition of human IDH1 R132H mutant expressed in IPTG-induced Escherichia coli BL21 cells assessed as oxidation of NADPH to NADP+ after 5 mins by spectrophotometry | Homo sapiens | 55 | ALA3632508 | cell-based format | Scientific Literature | |
6. | ALA3635922 | B | Inhibition of human IDH1 expressed in IPTG-induced Escherichia coli BL21 cells assessed as reduction of NADP+ to NADPH after 5 mins by spectrophotometry | Homo sapiens | 55 | ALA3632508 | cell-based format | Scientific Literature |