# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2050885 | B | Inhibition of mPGES-1 derived from IL1beta and TNFalpha stimulated human HeLa cells microsomal fraction using PGH2 as substrate at 1 uM preincubated for 30 mins prior to substrate addition measured after 1 min by LC-MS/MS analysis relative to control | Homo sapiens | 25 | ALA2046422 | cell-based format | Scientific Literature | |
2. | ALA2050886 | B | Inhibition of mPGES-1 derived from IL1beta and TNFalpha stimulated human HeLa cells microsomal fraction using PGH2 as substrate preincubated for 30 mins prior to substrate addition measured after 1 min by LC-MS/MS analysis | Homo sapiens | 9 | ALA2046422 | cell-based format | Scientific Literature | |
3. | ALA2050887 | B | Inhibition of mPGES-1 derived from LPS stimulated mouse RAW264.7 cells microsomal fraction using PGH2 as substrate at 1 uM preincubated for 30 mins prior to substrate addition measured after 1 min by LC-MS/MS analysis relative to control | Mus musculus | 9 | ALA2046422 | cell-based format | Scientific Literature | |
4. | ALA2050888 | B | Inhibition of mPGES-1 derived from LPS stimulated mouse NIH3T3 cells microsomal fraction using PGH2 as substrate at 1 uM preincubated for 30 mins prior to substrate addition measured after 1 min by LC-MS/MS analysis relative to control | Mus musculus | 9 | ALA2046422 | cell-based format | Scientific Literature | |
5. | ALA2050889 | B | Inhibition of ovine COX1-mediated PGE2 synthesis using PGH2 as substrate at 1 uM by LC-MS/MS analysis relative to control | Ovis aries | 9 | ALA2046422 | single protein format | Scientific Literature | |
6. | ALA2050890 | B | Inhibition of human recombinant COX2-mediated PGE2 synthesis using PGH2 as substrate at 1 uM by LC-MS/MS analysis relative to control | Homo sapiens | 9 | ALA2046422 | single protein format | Scientific Literature | |
7. | ALA2050891 | A | Cytotoxicity against IL1beta and TNFalpha-stimulated human HeLa cells at 1 to 10 uM after 24 hrs by WST1 assay | Homo sapiens | 8 | ALA2046422 | cell-based format | Scientific Literature | |
8. | ALA2050892 | B | Inhibition of mPGES-1 in IL1beta and TNFalpha-stimulated human HeLa cells assessed as PGE2 level after 24 hrs by LC-MS/MS analysis | Homo sapiens | 10 | ALA2046422 | cell-based format | Scientific Literature | |
9. | ALA2050893 | A | Cytotoxicity against IL1beta and TNFalpha-stimulated human HeLa cells at 100 uM after 24 hrs by WST1 assay | Homo sapiens | 4 | ALA2046422 | cell-based format | Scientific Literature | |
10. | ALA2050894 | B | Inhibition of mPGES-1 in IL1beta and TNFalpha-stimulated human HeLa cells assessed as PGE2 level at 1 uM after 24 hrs by LC-MS/MS analysis relative to control | Homo sapiens | 2 | ALA2046422 | cell-based format | Scientific Literature | |
11. | ALA2050895 | B | Inhibition of COX1 in human whole blood assessed as residual TXB2 level at 10 uM after 1 hr by LC-MS/MS analysis relative to control | Homo sapiens | 8 | ALA2046422 | single protein format | Scientific Literature | |
12. | ALA2050896 | B | Inhibition of COX1 in human whole blood assessed as residual PGD2 level at 10 uM after 1 hr by LC-MS/MS analysis relative to control | Homo sapiens | 8 | ALA2046422 | single protein format | Scientific Literature | |
13. | ALA2050899 | B | Inhibition of COX2 in LPS-stimulated human whole blood assessed as residual PGE2 level at 100 uM preincubated for 15 mins prior to LPS-stimulation measured after 24 hrs by LC-MS/MS analysis relative to control | Homo sapiens | 8 | ALA2046422 | single protein format | Scientific Literature | |
14. | ALA2050900 | B | Inhibition of 5-lipoxygenase/FLAP in calcium ionophore-stimulated human whole blood assessed as residual LTB4 level at 10 uM preincubated for 15 mins prior to calcium ionophore-stimulation measured after 15 mins by LC-MS/MS analysis | Homo sapiens | 8 | ALA2046422 | protein complex format | Scientific Literature | |
15. | ALA2050901 | B | Inhibition of COX2 in LPS-stimulated human whole blood assessed as residual PGF2alpha level at 100 uM preincubated for 15 mins prior to LPS-stimulation measured after 24 hrs by LC-MS/MS analysis relative to control | Homo sapiens | 8 | ALA2046422 | single protein format | Scientific Literature | |
16. | ALA2050992 | B | Inhibition of 5-lipoxygenase/FLAP in calcium ionophore-stimulated human whole blood assessed as residual 12(s)HETE level at 10 uM preincubated for 15 mins prior to calcium ionophore-stimulation measured after 15 mins by LC-MS/MS analysis | Homo sapiens | 8 | ALA2046422 | protein complex format | Scientific Literature | |
17. | ALA2050993 | B | Inhibition of 5-lipoxygenase/FLAP in calcium ionophore-stimulated human whole blood assessed as 5(s)HETE level at 10 uM preincubated for 15 mins prior to calcium ionophore-stimulation measured after 15 mins by LC-MS/MS analysis | Homo sapiens | 8 | ALA2046422 | protein complex format | Scientific Literature | |
18. | ALA2050994 | B | Inhibition of 5-lipoxygenase/FLAP in calcium ionophore-stimulated human whole blood assessed as residual 15(s)HETE level at 10 uM preincubated for 15 mins prior to calcium ionophore-stimulation measured after 15 mins by LC-MS/MS analysis | Homo sapiens | 8 | ALA2046422 | protein complex format | Scientific Literature | |
19. | ALA2050995 | B | Inhibition of 5-lipoxygenase/FLAP in calcium ionophore-stimulated human whole blood assessed as residual (+/-)11HETE level at 10 uM preincubated for 15 mins prior to calcium ionophore-stimulation measured after 15 mins by LC-MS/MS analysis | Homo sapiens | 8 | ALA2046422 | protein complex format | Scientific Literature | |
20. | ALA3606528 | B | Inhibition of mPGES-1 in IL-1beta-stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 formation incubated for 15 mins using PGH2 substrate by RP-HPLC method | Homo sapiens | 46 | ALA3603833 | cell-based format | Scientific Literature |