# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2066745 | F | Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level at 50 uM preincubated for 15 mins prior to LPS treatment by Griess method | Mus musculus | 9 | ALA2062500 | cell-based format | Scientific Literature | |
2. | ALA2066746 | A | Cytotoxicity in mouse RAW264.7 cells assessed as cell survival at 50 uM | Mus musculus | 9 | ALA2062500 | cell-based format | Scientific Literature | |
3. | ALA2066747 | F | Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method | Mus musculus | 10 | ALA2062500 | cell-based format | Scientific Literature | |
4. | ALA2066748 | B | Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells at 50 uM after 6 hrs by luciferase reporter gene assay | Homo sapiens | 9 | ALA2062500 | cell-based format | Scientific Literature | |
5. | ALA2066749 | A | Cytotoxicity in human HEK293 cells assessed as survival at 50 uM | Homo sapiens | 9 | ALA2062500 | cell-based format | Scientific Literature | |
6. | ALA2066750 | B | Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells after 6 hrs by luciferase reporter gene assay | Homo sapiens | 11 | ALA2062500 | cell-based format | Scientific Literature | |
7. | ALA3632013 | B | Inhibition of Stat3 dimer DNA binding activity in mouse NIH3T3/vSrc nuclear extract at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 24 | ALA3627612 | single protein format | Scientific Literature | |
8. | ALA3632014 | B | Inhibition of Stat1:Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | cell-based format | Scientific Literature | |
9. | ALA3632015 | B | Inhibition of Stat3 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | single protein format | Scientific Literature | |
10. | ALA3632016 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR assessed as residual activity at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 6 | ALA3627612 | single protein format | Scientific Literature | |
11. | ALA3632017 | B | Inhibition of Stat5 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe MGFe for 30 mins by EMSA | Mus musculus | 11 | ALA3627612 | cell-based format | Scientific Literature | |
12. | ALA3632018 | B | Inhibition of Stat1 dimer DNA binding activity in EGF-stimulated mouse NIH3T3 nuclear extract expressing human EGFR at 5 uM preincubated for 30 mins followed by addition of radiolabeled probe hSIE for 30 mins by EMSA | Mus musculus | 12 | ALA3627612 | single protein format | Scientific Literature | |
13. | ALA3632019 | B | Inhibition of Stat3 dimer DNA binding activity in human U251MG cells nuclear extract at 5 uM by EMSA using radiolabeled probe hSIE | Homo sapiens | 3 | ALA3627612 | cell-based format | Scientific Literature | |
14. | ALA3632020 | B | Inhibition of Stat3 dimer DNA binding activity in human U251MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE | Homo sapiens | 8 | ALA3627612 | cell-based format | Scientific Literature | |
15. | ALA3632021 | B | Inhibition of Stat3 dimer DNA binding activity in human U373MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE | Homo sapiens | 3 | ALA3627612 | cell-based format | Scientific Literature | |
16. | ALA3632022 | B | Inhibition of Stat3 activity in human U251MG cells assessed as phosphorylated Stat3 level at 5 uM by immunoblot analysis | Homo sapiens | 5 | ALA3627612 | cell-based format | Scientific Literature | |
17. | ALA3632023 | B | Inhibition of Stat3 activity in human U251MG cells assessed as phosphorylated Stat3 level after 2 hrs by immunoblot analysis | Homo sapiens | 8 | ALA3627612 | cell-based format | Scientific Literature | |
18. | ALA3632024 | B | Inhibition of Stat3 activity in human glioma (G22) patient-derived mouse xenograft cells assessed as phosphorylated Stat3 level at 5 uM after 3 hrs by immunoblot analysis | Homo sapiens | 3 | ALA3627612 | assay format | Scientific Literature | |
19. | ALA3632025 | B | Inhibition of Stat3 activity in human glioma (G22) patient-derived mouse xenograft cells assessed as phosphorylated Stat3 level at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 3 | ALA3627612 | assay format | Scientific Literature | |
20. | ALA3632026 | F | Cytotoxicity against human Glioma (G22) patient-derived mouse xenograft cells at 5 uM after 24 hrs by cyquant assay | Homo sapiens | 3 | ALA3627612 | cell-based format | Scientific Literature |