# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2114835 | F | PubChem BioAssay. qHTS assay to identify small molecules that stimulate interleukin-8 (IL-8) secretion. (Class of assay: confirmatory) | Homo sapiens | 321 | assay format | PubChem BioAssays | ||
2. | ALA3283234 | B | Inhibition of Holtzman rat brain choline acetyltransferase using [1-14C-acetyl]-CoA as substrate at 1 mM incubated in un-buffered water for 24 hrs by liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
3. | ALA3283235 | P | Stability in aqueous medium by NMR spectroscopy | 1 | small-molecule physicochemical format | Scientific Literature | |||
4. | ALA3283236 | P | Stability in D2O assessed every 4 mins for 1 hr by NMR spectroscopy | 1 | small-molecule physicochemical format | Scientific Literature | |||
5. | ALA3283237 | P | Stability in D2O by NMR spectroscopy | 1 | small-molecule physicochemical format | Scientific Literature | |||
6. | ALA3283238 | P | Stability in Me2SO-d6 by NMR spectroscopy | 1 | small-molecule physicochemical format | Scientific Literature | |||
7. | ALA3283239 | P | Stability in chloroform-d by NMR spectroscopy | 1 | small-molecule physicochemical format | Scientific Literature | |||
8. | ALA3283240 | B | Inhibition of Holtzman rat brain choline acetyltransferase using [1-14C-acetyl]-CoA as substrate at 1 mM treated with buffered solution at pH 7.4 for 3 to 60 mins prior to enzyme addition followed by 20 mins incubation prior to substrate addition measured after 1 hr by liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
9. | ALA3283242 | B | Inhibition of Holtzman rat brain choline acetyltransferase using [1-14C-acetyl]-CoA as substrate incubated 20 mins prior to substrate addition measured after 30 mins by liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
10. | ALA3283243 | B | Reversible inhibition of Holtzman rat brain choline acetyltransferase using [1-14C-acetyl]-CoA as substrate at 5 X 10'-4 M incubated 20 mins prior to substrate addition measured after 30 mins by liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
11. | ALA3283244 | B | Uncompetitive inhibition of Holtzman rat brain choline acetyltransferase using choline as substrate at 1 mM incubated 20 mins prior to substrate addition measured after 30 mins by LB plot using liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
12. | ALA3283245 | B | Uncompetitive inhibition of Holtzman rat brain choline acetyltransferase using acetyl-CoA as substrate at 0.2 mM incubated 20 mins prior to substrate addition measured after 30 mins by LB plot using liquid scintillation counting analysis | Rattus norvegicus | 1 | assay format | Scientific Literature | ||
13. | ALA3283246 | P | Stability of the compound assessed as autohydrolysis at 5 X 10'-3 M at pH 7.4 | 2 | small-molecule physicochemical format | Scientific Literature | |||
14. | ALA3283247 | A | Stability of the compound assessed as acetylcholinesterase (unknown origin)-mediated hydrolysis at 5 X 10'-3 M | Homo sapiens | 2 | single protein format | Scientific Literature | ||
15. | ALA3283248 | A | Stability of the compound assessed as butyrylcholine esterase (unknown origin)-mediated hydrolysis at 5 X 10'-3 M | Homo sapiens | 2 | single protein format | Scientific Literature | ||
16. | ALA3283249 | B | Inhibition of choline acetyltransferase in frog sciatic nerve-gastrocnemius muscle assessed as blockade of electrically stimulated muscle contraction after 3 hrs | Lithobates pipiens | 1 | tissue-based format | Scientific Literature | ||
17. | ALA3283523 | B | Inhibition of choline acetyltransferase in guinea pig ileum assessed as blockade of electrically stimulated muscle contraction after 3 hrs | Cavia porcellus | 1 | tissue-based format | Scientific Literature | ||
18. | ALA3283524 | B | Inhibition of choline acetyltransferase in frog sciatic nerve-gastrocnemius muscle assessed as blockade of electrically stimulated muscle contraction at 10'-3 M incubated for 3 hrs followed by compound washout | Lithobates pipiens | 1 | tissue-based format | Scientific Literature | ||
19. | ALA3283525 | P | Dissociation constant, pKa of the compound | 1 | small-molecule physicochemical format | Scientific Literature | |||
20. | ALA3283526 | P | Half life of the compound auto-hydrolysis at 5 X 10'-3 M at pH 7.4 | 1 | small-molecule physicochemical format | Scientific Literature |