# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2148625 | B | Inhibition of human ROMK1 channel expressed in CHO cells coexpressing DHFR assessed as inhibition of 86Rb+ efflux after 35 mins by TopCount method | Homo sapiens | 36 | ALA2146432 | cell-based format | Scientific Literature | |
2. | ALA2148626 | B | Displacement of [35S]MK499 from human ERG | Homo sapiens | 30 | ALA2146432 | single protein format | Scientific Literature | |
3. | ALA2148627 | B | Inhibition of Kir2.1 expressed in HEK293 cells assessed as inhibition of thallium efflux by fluorescence assay | Homo sapiens | 2 | ALA2146432 | cell-based format | Scientific Literature | |
4. | ALA2148628 | B | Inhibition of human Kir2.3 expressed in CHO cells assessed as inhibition of 86Rb+ efflux | Homo sapiens | 2 | ALA2146432 | cell-based format | Scientific Literature | |
5. | ALA2148629 | B | Selectivity ratio of IC50 for human ERG to IC50 for human ROMK1 | Homo sapiens | 2 | ALA2146432 | assay format | Scientific Literature | |
6. | ALA2148630 | B | Inhibition of ROMK by electrophysiology assay | 3 | ALA2146432 | single protein format | Scientific Literature | ||
7. | ALA2148631 | B | Inhibition of Kir4.1 expressed in HEK293 cells assessed as inhibition of thallium efflux up to 100 uM by fluorescence assay | Homo sapiens | 3 | ALA2146432 | cell-based format | Scientific Literature | |
8. | ALA2148632 | B | Inhibition of Kir7.1 expressed in HEK293 cells assessed as inhibition of thallium efflux up to 100 uM by fluorescence assay | Homo sapiens | 3 | ALA2146432 | cell-based format | Scientific Literature | |
9. | ALA2148633 | A | Clearance in rat at 1 mg/kg, iv | Rattus norvegicus | 2 | ALA2146432 | organism-based format | Scientific Literature | |
10. | ALA2148634 | A | Half life in rat at 1 mg/kg, iv | Rattus norvegicus | 2 | ALA2146432 | organism-based format | Scientific Literature | |
11. | ALA2148635 | A | Dose normalised AUC in rat at 2 mg/kg, po | Rattus norvegicus | 2 | ALA2146432 | organism-based format | Scientific Literature | |
12. | ALA2148636 | A | Oral bioavailability in rat at 2 mg/kg | Rattus norvegicus | 2 | ALA2146432 | organism-based format | Scientific Literature | |
13. | ALA3705830 | B | Thallium Flux Assay: FluxOR Kit Components (Invitrogen F10017) FluxOR Reagent (Component A) FluxOR Assay Buffer (Component B)-10x Concentrate PowerLoad Concentrate (Component C)-100x Concentrate Probenecid (Component D)-Lyophilized sample is kept at -20 C. Water soluble, 100x after solubilization in 1 ml water. Store at 4 C. FluxOR Chloride-free Buffer (Component E)-5x Concentrate Potassium sulfate (K2SO4) Concentrate (Component F)-125 mM in water. Store at 4 C. Thallium sulfate (Tl2SO4) Concentrate (Component G)-50 mM in water. Store at 4 C. DMSO (dimethyl sulfoxide, Component H)-1 ml (100%) Reagent Preparation- FluxOR Working Solutions 1000x FluxOR Reagent: Reconstitute a vial of component A in 100 ul DMSO; Mix well; Store 10 ul aliquots at -20 C. 1x FluxOR Assay Buffer: Dilute Component B 10-fold with water; Adjust pH to 7.4 with Hepes/NaOH; Filter and store at 4 C. | Homo sapiens | 9 | ALA3639123 | single protein format | BindingDB Database | |
14. | ALA4427467 | B | Inhibition of ROMK (unknown origin) after 30 mins by 86Rb+ efflux assay | Homo sapiens | 13 | ALA4425169 | single protein format | Scientific Literature | |
15. | ALA4427468 | B | Displacement of [35S]MK-499 from human ERG | Homo sapiens | 13 | ALA4425169 | single protein format | Scientific Literature | |
16. | ALA4427469 | B | Selectivity ratio of IC50 for human ERG to IC50 for ROMK (unknown origin) | Homo sapiens | 10 | ALA4425169 | assay format | Scientific Literature |