#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3783933	Binding	Inhibition of human CYP17A1 expressed in HEK293 cell microsomes using [3H]-pregnenolone as substrate incubated for 45 mins by scintillation proximity assay in presence of NADPH	Homo sapiens	12	ALA3779801	cell-based format	Scientific Literature
2.	ALA3783934	ADME	Inhibition of CYP1A2 (unknown origin)	Homo sapiens	12	ALA3779801	single protein format	Scientific Literature
3.	ALA3783935	ADME	Metabolic stability in human liver microsomes assessed as parent compound remaining at 0.5 uM incubated for 10 mins	Homo sapiens	12	ALA3779801	tissue-based format	Scientific Literature
4.	ALA3783936	ADME	Metabolic stability in mouse liver microsomes assessed as parent compound remaining at 0.5 uM incubated for 10 mins	Mus musculus	12	ALA3779801	tissue-based format	Scientific Literature
5.	ALA3783937	ADME	Metabolic stability in cynomolgus monkey liver microsomes assessed as parent compound remaining at 0.5 uM incubated for 10 mins	Macaca fascicularis	12	ALA3779801	tissue-based format	Scientific Literature
6.	ALA3784028	Binding	Selectivity ratio of human CYP17A1 hydroxylase activity expressed in Escherichia coli using pregnenolone substrate to human CYP17A1 lyase activity expressed in HEK293 cell microsomes using [3H]-hydroxypregnenolone substrate	Homo sapiens	6	ALA3779801	cell-based format	Scientific Literature
7.	ALA2150657	Binding	Inhibition of CYP17 transfected in human HEK2 cells using [3H]pregnenolone as substrate by SPA assay	Homo sapiens	14	ALA2146479	cell-based format	Scientific Literature
8.	ALA3887255	Binding	SPA Assay: The assays were performed in U-bottom 384-well optiplates. The final assay volume was 15 ul prepared from 7.5 ul additions of microsomes (prepared as a high-speed pellet from homogenized HEK2 cells stably transfected with CYP17), substrates (3H-Pregnenolone and NADPH) and test compounds in assay buffer (50 mM Potassium phosphate pH 7.2, 10% glycerol). The reaction was initiated by the combination of the microsomes and substrates in wells containing compound. The reaction was incubated at room temperature for 45 minutes and terminated by adding 7.5 ul of 0.2N HCl to each well. Following an incubation period of 10 minutes, anti-DHEA-coated SPA beads were added to the terminated reaction. The plate was sealed and incubated overnight with shaking at 4 C. The beads were allowed to settle in the plate for 1 hour and the plate read on a TOPCOUNT (Perkin-Elmer) plate reader.Inhibition data were calculated by comparison to no enzyme control reactions for 100% inhibition.	Homo sapiens	15	ALA3886242	cell-based format	BindingDB Database