Inhibition of Mycobacterium tuberculosis Rv0183 assessed as covalent bond formation with enzyme serine residue by measuring change in molecular weight of enzyme-compound complexes at 40 molar excess compound concentration pre-incubated for 30 mins with enzyme before substrate addition by MALDI-TOF mass spectrometry method
Inhibition of dog recombinant gastric lipase expressed in transgenic maize at 200 molar excess compound concentration pre-incubated for 2 hrs with enzyme before substrate addition by pH-stat technique
Inhibition of guinea pig recombinant PLRP2 expressed in Aspergillus oryzae at 200 molar excess compound concentration pre-incubated for 2 hrs with enzyme before substrate addition by pH-stat technique
Inhibition of human recombinant pancreatic lipase expressed in Pichia pastoris at 200 molar excess compound concentration pre-incubated for 2 hrs with enzyme before substrate addition by pH-stat technique
Inhibition of Mycobacterium tuberculosis Rv0183 assessed as half inactivation time pre-incubated with enzyme before substrate addition by pH-stat technique
Inhibition of Mycobacterium tuberculosis Rv0183 assessed as residual enzyme activity pre-incubated for 30 mins with enzyme before substrate addition by pH-stat technique
Inhibition of Mycobacterium tuberculosis triacylglycerol lipase LipY at 20 molar excess compound concentration preincubated with enzyme measured after 30 mins by pH-stat technique relative to control
Inhibition of Mycobacterium tuberculosis monoacylglycerol lipase Rv0183 at 20 molar excess compound concentration preincubated with enzyme measured after 30 mins by pH-stat technique relative to control
Inhibition of Fusarium solani pisi cutinase at 20 molar excess compound concentration preincubated with enzyme measured after 30 mins by pH-stat technique relative to control
Antibacterial activity against Mycobacterium tuberculosis H37Rv expressing GFP infected in mouse Raw264.7 cells incubated for 5 days by SYTO 60 staining method