Activity at muscarinic M4 receptor expressing in CHONFAT cells assessed as potentiation of acetylcholine-induced calcium mobilization preincubated for 4 mins and measured 4 mins after acetylcholine stimulation by FLIPR assay
Activity at muscarinic M3 receptor expressing in CHONFAT cells assessed as potentiation of acetylcholine-induced calcium mobilization preincubated for 4 mins and measured 4 mins after acetylcholine stimulation by FLIPR assay
Activity at muscarinic M2 receptor expressing in CHONFAT cells assessed as potentiation of acetylcholine-induced calcium mobilization preincubated for 4 mins and measured 4 mins after acetylcholine stimulation by FLIPR assay
Positive allosteric modulation of human muscarinic M1 receptor expressing in CHONFAT cells assessed as potentiation of acetylcholine-induced calcium mobilization preincubated for 4 mins and measured 4 mins after acetylcholine stimulation by FLIPR assay