# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2319007 | Functional | Growth inhibition of human SF539 cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
2. | ALA2319009 | Functional | Growth inhibition of human MDA-MB-435 cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
3. | ALA2319010 | Functional | Growth inhibition of human SN12C cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
4. | ALA2319011 | Functional | Growth inhibition of human DU145 cells | Homo sapiens | 15 | ALA2311405 | cell-based format | Scientific Literature | |
5. | ALA2319012 | Functional | Growth inhibition of human OVCAR3 cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
6. | ALA2319013 | Functional | Growth inhibition of human UACC62 cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
7. | ALA2319014 | Functional | Growth inhibition of human HCT116 cells | Homo sapiens | 16 | ALA2311405 | cell-based format | Scientific Literature | |
8. | ALA2319015 | Functional | Growth inhibition of human HOP62 cells | Homo sapiens | 15 | ALA2311405 | cell-based format | Scientific Literature | |
9. | ALA2319019 | Binding | Inhibition of recombinant Tdp1 (unknown origin) using 5'-[32P]-labeled single-stranded DNA oligonucleotide containing 3'-phosphotyrosine as substrate after 15 mins by PAGE analysis | Homo sapiens | 50 | ALA2311405 | single protein format | Scientific Literature | |
10. | ALA2330501 | Binding | Binding affinity to RXRalpha (unknown origin) at 10 uM after 2 hrs by ultrafiltration LC-MS/MS analysis relative to control | Homo sapiens | 6 | ALA2321659 | single protein format | Scientific Literature | |
11. | ALA2330502 | Binding | Activation of human RXRalpha activity expressed in COS1 cells assessed as induction ratio after 12 hrs by RXRE-luciferase reporter gene assay relative to untreated control | Homo sapiens | 35 | ALA2321659 | cell-based format | Scientific Literature | |
12. | ALA2330503 | ADME | Cytotoxicity against African green monkey COS1 cells assessed as cell survival | Chlorocebus aethiops | 36 | ALA2321659 | cell-based format | Scientific Literature | |
13. | ALA2330504 | Binding | Activation of human RXRalpha activity expressed in COS1 cells after 12 hrs by RXRE-luciferase reporter gene assay | Homo sapiens | 39 | ALA2321659 | cell-based format | Scientific Literature | |
14. | ALA2330505 | Binding | Activation of human RXRalpha activity expressed in COS1 cells assessed as induction ratio at 50 uM after 12 hrs by RXRE-luciferase reporter gene assay relative to untreated control | Homo sapiens | 113 | ALA2321659 | cell-based format | Scientific Literature | |
15. | ALA3888763 | Binding | Gel-based Assay : A 5′-[32P]-labeled single-stranded DNA oligonucleotide containing a 3′-phosphotyrosine (N14Y) was generated as described by Dexheimer et al. The DNA substrate was then incubated with 5 pM recombinant Tdp1 in the absence or presence of inhibitor for 15 min at room temperature in a buffer containing 50 mM Tris HCl, pH 7.5, 80 mM KCl, 2 mM EDTA, 1 mM DTT, 40 μg/ml BSA and 0.01% Tween-20. Reactions were terminated by the addition of 1 volume of gel loading buffer [99.5% (v/v) formamide, 5 mM EDTA, 0.01% (w/v) xylene cyanol, and 0.01% (w/v) bromophenol blue]. Samples were subjected to a 16% denaturing PAGE and gels were exposed after drying to a Phosphorlmager screen (GE Healthcare). Gel images were scanned using a Typhoon 8600 (GE Healthcare) and densitometric analyses were performed using the ImageQuant software (GE Healthcare). | 12 | ALA3886826 | single protein format | BindingDB Database |