#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2330500	B	Binding affinity to VDR (unknown origin) after 30 mins by ultrafiltration LC-MS/MS analysis relative to 6-(6-aminohexyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione	Homo sapiens	10		single protein format	Scientific Literature
2.	ALA2330501	B	Binding affinity to RXRalpha (unknown origin) at 10 uM after 2 hrs by ultrafiltration LC-MS/MS analysis relative to control	Homo sapiens	6		single protein format	Scientific Literature
3.	ALA2330502	B	Activation of human RXRalpha activity expressed in COS1 cells assessed as induction ratio after 12 hrs by RXRE-luciferase reporter gene assay relative to untreated control	Homo sapiens	35		cell-based format	Scientific Literature
4.	ALA2330503	A	Cytotoxicity against African green monkey COS1 cells assessed as cell survival	Chlorocebus aethiops	36		cell-based format	Scientific Literature
5.	ALA2330504	B	Activation of human RXRalpha activity expressed in COS1 cells after 12 hrs by RXRE-luciferase reporter gene assay	Homo sapiens	39		cell-based format	Scientific Literature
6.	ALA2330505	B	Activation of human RXRalpha activity expressed in COS1 cells assessed as induction ratio at 50 uM after 12 hrs by RXRE-luciferase reporter gene assay relative to untreated control	Homo sapiens	113		cell-based format	Scientific Literature
7.	ALA3706179	B	Gel-Based Assay: Tdp1 reactions were performed as recently described. Briefly, a 5'-[32P]-labeled single-stranded DNA oligonucleotide containing a 3'-phosphotyrosine (N14Y) incubated with 5 pM recombinant Tdp1 in the absence or presence of inhibitor for 15 min at room temperature in a buffer containing 50 mM Tris HCl, pH 7.5, 80 mM KCl, 2 mM EDTA, 1 mM DTT, 40 ug/ml BSA and 0.01% Tween-20. Reactions were terminated by the addition of 1 volume of gel loading buffer [99.5% (v/v) formamide, 5 mM EDTA, 0.01% (w/v) xylene cyanol, and 0.01% (w/v) bromophenol blue]. Samples were subjected to a 16% denaturing PAGE and dried gels were exposed to a PhosphorImager screen (GE Healthcare). Gel images were scanned using a Typhoon 8600 (GE Healthcare) and densitometric analyses were performed using the ImageQuant software (GE Healthcare).Binding experiments were performed as recently described. Briefly, Tdp1 was amine coupled to a CM5 sensor chip (GE Healthcare, Piscataway N.J.).		12		single protein format	BindingDB Database
8.	ALA3888763	B	Gel-based Assay : A 5′-[32P]-labeled single-stranded DNA oligonucleotide containing a 3′-phosphotyrosine (N14Y) was generated as described by Dexheimer et al. The DNA substrate was then incubated with 5 pM recombinant Tdp1 in the absence or presence of inhibitor for 15 min at room temperature in a buffer containing 50 mM Tris HCl, pH 7.5, 80 mM KCl, 2 mM EDTA, 1 mM DTT, 40 μg/ml BSA and 0.01% Tween-20. Reactions were terminated by the addition of 1 volume of gel loading buffer [99.5% (v/v) formamide, 5 mM EDTA, 0.01% (w/v) xylene cyanol, and 0.01% (w/v) bromophenol blue]. Samples were subjected to a 16% denaturing PAGE and gels were exposed after drying to a Phosphorlmager screen (GE Healthcare). Gel images were scanned using a Typhoon 8600 (GE Healthcare) and densitometric analyses were performed using the ImageQuant software (GE Healthcare).		12		single protein format	BindingDB Database