# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2345505 | B | Inhibition of chymotrypsin-like activity of yeast 20S proteasome beta-5 subunit using Suc-LLVY-AMC as substrate measured up to 45 mins by fluorescence assay | Saccharomyces cerevisiae | 2 | ALA2331312 | single protein format | Scientific Literature | |
2. | ALA2345506 | P | Half life of the compound in RPMI culture medium in presence of 20% fetal calf serum at 37 degC by HPLC analysis | 3 | ALA2331312 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA2345507 | B | Inhibition of human cathepsin B using Suc-LLVY-AMC as substrate assessed as remaining enzyme activity at 20 uM incubated for 15 mins prior to substrate addition measured for 30 mins by fluorescence assay | Homo sapiens | 11 | ALA2331312 | single protein format | Scientific Literature | |
4. | ALA2345508 | B | Inhibition of human calpain 1 using Suc-LLVY-AMC as substrate assessed as remaining enzyme activity at 20 uM incubated for 5 mins prior to substrate addition measured for 30 mins by fluorescence assay | Homo sapiens | 11 | ALA2331312 | single protein format | Scientific Literature | |
5. | ALA2345511 | B | Inhibition of postacid activity of 20s proteasome beta-1 subunit in HEK293 cells using Z-nLPnLD-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta1 assay | Homo sapiens | 4 | ALA2331312 | cell-based format | Scientific Literature | |
6. | ALA2345512 | B | Inhibition of chymotrypsin-like activity of proteasome beta-5 subunit in HEK293 cells using Suc-LLVY-Glo as substrate incubated for 2 hrs prior to substrate addition measured after 10 mins by cell-based proteasome-Glo beta5 assay | Homo sapiens | 8 | ALA2331312 | cell-based format | Scientific Literature | |
7. | ALA2345514 | B | Mixed type inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as inhibition constant for dissociation of enzyme-substrate-inhibitor complex incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 3 | ALA2331312 | single protein format | Scientific Literature | |
8. | ALA2345517 | B | Mixed type inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as inhibition constant for dissociation of enzyme-inhibitor complex incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 3 | ALA2331312 | single protein format | Scientific Literature | |
9. | ALA2345964 | B | Inhibition of trypsin-like activity of human constitutive 20s proteasome beta-2 subunit using Boc-LRR-AMC as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
10. | ALA2345965 | B | Inhibition of postacid activity of human constitutive 20s proteasome beta-1 subunit using Z-LLE-betaNA as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
11. | ALA2343600 | B | Inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
12. | ALA3388361 | B | Inhibition of chymotrypsin-like activity of human constitutive 20S proteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
13. | ALA3388362 | B | Inhibition of PA activity of human constitutive 20S proteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
14. | ALA3388363 | B | Inhibition of trypsin-like activity of human constitutive 20S proteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
15. | ALA3388364 | B | Inhibition of chymotrypsin-like activity of human inducible 20S immunoproteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
16. | ALA3388365 | B | Inhibition of PA activity of human inducible 20S immunoproteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
17. | ALA3388366 | B | Inhibition of trypsin-like activity of human inducible 20S immunoproteasome incubated for 15 mins before fluorogenic substrate addition | Homo sapiens | 5 | ALA3351843 | assay format | Scientific Literature | |
18. | ALA4255085 | F | Cytotoxicity against human HuH7 cells assessed as reduction in cell viability after 48 hrs by Hoechst 33342 staining based HCS assay | Homo sapiens | 6 | ALA4251654 | cell-based format | Scientific Literature | |
19. | ALA4255086 | F | Cytotoxicity against human Caco2 cells assessed as reduction in cell viability after 48 hrs by Hoechst 33342 staining based HCS assay | Homo sapiens | 6 | ALA4251654 | cell-based format | Scientific Literature | |
20. | ALA4255087 | F | Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by Hoechst 33342 staining based HCS assay | Homo sapiens | 6 | ALA4251654 | cell-based format | Scientific Literature |