# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2345505 | B | Inhibition of chymotrypsin-like activity of yeast 20S proteasome beta-5 subunit using Suc-LLVY-AMC as substrate measured up to 45 mins by fluorescence assay | Saccharomyces cerevisiae | 2 | ALA2331312 | single protein format | Scientific Literature | |
2. | ALA2345507 | B | Inhibition of human cathepsin B using Suc-LLVY-AMC as substrate assessed as remaining enzyme activity at 20 uM incubated for 15 mins prior to substrate addition measured for 30 mins by fluorescence assay | Homo sapiens | 11 | ALA2331312 | single protein format | Scientific Literature | |
3. | ALA2345508 | B | Inhibition of human calpain 1 using Suc-LLVY-AMC as substrate assessed as remaining enzyme activity at 20 uM incubated for 5 mins prior to substrate addition measured for 30 mins by fluorescence assay | Homo sapiens | 11 | ALA2331312 | single protein format | Scientific Literature | |
4. | ALA2345513 | B | Noncompetitive inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as inhibition constant for dissociation of enzyme-substrate-inhibitor complex incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 1 | ALA2331312 | single protein format | Scientific Literature | |
5. | ALA2345516 | B | Noncompetitive inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as inhibition constant for dissociation of enzyme-inhibitor complex incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 1 | ALA2331312 | single protein format | Scientific Literature | |
6. | ALA2345964 | B | Inhibition of trypsin-like activity of human constitutive 20s proteasome beta-2 subunit using Boc-LRR-AMC as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
7. | ALA2345965 | B | Inhibition of postacid activity of human constitutive 20s proteasome beta-1 subunit using Z-LLE-betaNA as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
8. | ALA2343600 | B | Inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 12 | ALA2331312 | single protein format | Scientific Literature | |
9. | ALA4255067 | B | Inhibition of human 20S constitutive proteasome beta-1 using Z-LLE-betaNA as substrate incubated for 15 mins measured for 45 mins by fluorescence assay | Homo sapiens | 22 | ALA4251654 | single protein format | Scientific Literature | |
10. | ALA4255068 | B | Inhibition of human 20S immunoproteasome beta-1 using Ac-PAL-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay | Homo sapiens | 24 | ALA4251654 | single protein format | Scientific Literature | |
11. | ALA4255069 | B | Inhibition of human 20S constitutive proteasome beta-1 at 50 uM using Z-LLE-betaNA as substrate incubated for 15 mins measured for 45 mins by fluorescence assay relative to control | Homo sapiens | 31 | ALA4251654 | single protein format | Scientific Literature | |
12. | ALA4255070 | B | Inhibition of human 20S immunoproteasome beta-1 at 50 uM using Ac-PAL-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay relative to control | Homo sapiens | 31 | ALA4251654 | single protein format | Scientific Literature | |
13. | ALA4255071 | B | Inhibition of human 20S constitutive proteasome beta-2 using BocLRR-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay | Homo sapiens | 13 | ALA4251654 | single protein format | Scientific Literature | |
14. | ALA4255072 | B | Inhibition of human 20S immunoproteasome beta-2 using BocLRR-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay | Homo sapiens | 16 | ALA4251654 | single protein format | Scientific Literature | |
15. | ALA4255073 | B | Inhibition of human 20S constitutive proteasome beta-2 at 50 uM using BocLRR-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay relative to control | Homo sapiens | 31 | ALA4251654 | single protein format | Scientific Literature | |
16. | ALA4255074 | B | Inhibition of human 20S immunoproteasome beta-2 at 50 uM using BocLRR-AMC as substrate incubated for 15 mins measured for 45 mins by fluorescence assay relative to control | Homo sapiens | 31 | ALA4251654 | single protein format | Scientific Literature | |
17. | ALA4255075 | B | Inhibition of yeast 20S constitutive proteasome beta-5 | Saccharomyces cerevisiae | 4 | ALA4251654 | assay format | Scientific Literature | |
18. | ALA4255076 | B | Inhibition of yeast 20S immunoproteasome beta-5 | Saccharomyces cerevisiae | 3 | ALA4251654 | assay format | Scientific Literature |