Assay

#	Aladdin ID	Assay Type	Description	Organism	Compounds	BAO Format	Source
1.	ALA2343130	B	Inhibition of rat full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Rattus norvegicus	20	cell-based format	Scientific Literature
2.	ALA2343131	B	Inhibition of mouse full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Mus musculus	20	cell-based format	Scientific Literature
3.	ALA2343132	B	Inhibition of human full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Homo sapiens	20	cell-based format	Scientific Literature
4.	ALA2343135	B	Inhibition of human recombinant DAAO after 30 mins by plate reader analysis	Homo sapiens	26	single protein format	Scientific Literature
5.	ALA3888090	B	In Vitro DAAO Enzyme Assay: Human DAAO enzyme was supplied by the Takeda Pharmaceutical Company (Osaka) and each batch was tested and used at concentrations giving comparable levels of activity. The Km of D-Serine was measured for each enzyme batch to maintain consistency; this Km was used in subsequent assays.On the day of the assay compounds were serially diluted in DMSO before being diluted 1:20 with assay buffer (20 mM Tris ph 7.4). A 5 ul portion of assay buffer was added to the wells of a 384 clear base black-walled plate (Corning), 5 ul of diluted compound was then added via automated plate to plate transfer using the Bravo liquid handler (Agilent technologies) followed by 5 ul of human DAAO enzyme and then 5 ul D-Serine 50 mM was added to all but the negative control wells (final concentration of 10 mM). Finally 5 ul Amplex red reagent (Invitrogen) was added to all wells as per manufacturer's protocol. The plate was incubated for 60 minutes in the dark at 25° C., and the fluorescence in each well was measured in the Envision plate reader.	Homo sapiens	66	single protein format	BindingDB Database