#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2343130	B	Inhibition of rat full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Rattus norvegicus	20		cell-based format	Scientific Literature
2.	ALA2343131	B	Inhibition of mouse full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Mus musculus	20		cell-based format	Scientific Literature
3.	ALA2343132	B	Inhibition of human full length DAAO overexpressed in HEK293 cells after 30 mins by plate reader analysis	Homo sapiens	20		cell-based format	Scientific Literature
4.	ALA2343135	B	Inhibition of human recombinant DAAO after 30 mins by plate reader analysis	Homo sapiens	26		single protein format	Scientific Literature
5.	ALA3887715	B	Inhibition Assay: The DAAO inhibitory activity was measured by assaying the amount of hydrogen peroxide (H2O2) produced by reacting DAAO protein with flavin adenine dinucleotide (FAD) and D-alanine. The amount of H2O2 was determined by measuring the fluorescence generated on conversion of Amplex red (manufactured by Invitrogen Co.) into resorufin by the reaction of H2O2 with horseradish peroxidase (HRP). 4 uL of 4% dimethyl sulfoxide (DMSO) buffer (50 mM sodium phosphate (pH 7.5), 0.02% CHAPS) solution of the test compound was added to 384-well black, low volume plate, a mixed solution (4 uL) of recombinant human DAAO protein (15 nM), which had been expressed in Escherichia coli and purified, and 18 uM FAD was added, and the mixture was incubated at room temperature for 15 min. After incubation, a mixed buffer (4 uL) of 2.25 mM D-alanine, 1.5 U/mL HRP and 150 uM Amplex red was added, the mixture was incubated at room temperature for 30 min.	Homo sapiens	34		assay format	BindingDB Database
6.	ALA3887716	B	Inhibition Assay: This test was performed by partially modifying the method of Philip et. al. (J. Biomol. Screen. Vol. 11, pp 481-487, 2006). HEK293 cells that stably express human DAAO were suspended in Cellbanker solution at 5×106 cells/ml and cryopreserved at −80° C. At the time of measurement, the cells were centrifuged at 1000 rpm for one min and the Cellbanker solution was removed. The cells were resuspended at 5×106 cells/ml in FAD-containing buffer (50 mM sodium phosphate [pH 7.5], 18 μM FAD, 0.02% CHAPS). A 4% DMSO buffer solution (50 mM sodium phosphate [pH 7.5], 0.02% CHAPS) of the test compound (4 μL) was added to a 384-well black, low volume plate, the cell suspension (4 μL) was added, and the mixture was incubated at room temperature for 15 min. After incubation, a mixed buffer (4 μL) of 150 mM D-alanine, 1.5 U/mL HRP and 240 μM Amplex red was added to the plate, and the mixture was incubated at room temperature for 30 min, and the fluorescence (excitation w	Homo sapiens	30		cell-based format	BindingDB Database