#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2351227	F	Selectivity index, ratio of IC50 for human HS68 cells to IC50 for human A549 cells	Homo sapiens	7	ALA2346500	cell-based format	Scientific Literature
2.	ALA2351228	F	Selectivity index, ratio of IC50 for mouse MEF cells to IC50 for human A549 cells		7	ALA2346500	cell-based format	Scientific Literature
3.	ALA2351229	A	Cytotoxicity against human HS68 cells after 72 hrs by MTT assay	Homo sapiens	7	ALA2346500	cell-based format	Scientific Literature
4.	ALA2351230	A	Cytotoxicity against mouse MEF cells after 72 hrs by MTT assay	Mus musculus	7	ALA2346500	cell-based format	Scientific Literature
5.	ALA2351231	F	Cytotoxicity against human A549 cells after 72 hrs by MTT assay	Homo sapiens	7	ALA2346500	cell-based format	Scientific Literature
6.	ALA2351232	B	Inhibition of HDAC6 in human A549 cells assessed as increase in tubulin acetylation after 24 hrs by Western blotting analysis	Homo sapiens	6	ALA2346500	cell-based format	Scientific Literature
7.	ALA2351233	B	Inhibition of class 1 HDAC in human A549 cells assessed as increase in histone acetylation after 24 hrs by Western blotting analysis	Homo sapiens	6	ALA2346500	cell-based format	Scientific Literature
8.	ALA2351234	B	Inhibition of HMG-CoA reductase in human A549 cells after 5 mins by spectrophotometric analysis	Homo sapiens	9	ALA2346500	cell-based format	Scientific Literature
9.	ALA2351235	B	Inhibition of recombinant HDAC6 (unknown origin) after 10 mins by fluorimetric analysis	Homo sapiens	10	ALA2346500	single protein format	Scientific Literature
10.	ALA2351477	B	Inhibition of recombinant HDAC2 (unknown origin) after 10 mins by fluorimetric analysis	Homo sapiens	10	ALA2346500	single protein format	Scientific Literature
11.	ALA2351478	B	Inhibition of recombinant HDAC1 (unknown origin) after 10 mins by fluorimetric analysis	Homo sapiens	10	ALA2346500	single protein format	Scientific Literature
12.	ALA2351479	B	Inhibition of recombinant HMG-CoA reductase (unknown origin) after 10 mins by spectrophotometric analysis	Homo sapiens	10	ALA2346500	single protein format	Scientific Literature
13.	ALA3888526	B	Reductase Activity Assay: The HMGR activity was performed using HMG-CoA reductase assay kit from Sigma-Aldrich with the human recombinant protein or 100 μg total cell lysates from A549 cells. Lovastatin was used as a positive control, and SAHA as a negative control. HMGR activity under defined assay conditions, containing NADPH and HMG-CoA substrate in a final volume of 0.2 mL of 100 mM potassium phosphatate buffer (120 mM KCl, 1 mM EDTA, 5 mM DTT, pH 7.4), were initiated in the presence or absence (control) of test compounds dissolved in dimethylsulfoxide (DMSO). The rates of NADPH consumption were monitored every 20 seconds, for up to 10 minutes, by spectrophotometer at 37° C. and 340 nm.		7	ALA3886749	cell-based format	BindingDB Database
14.	ALA3888527	B	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	Homo sapiens	7	ALA3886749	single protein format	BindingDB Database
15.	ALA3888528	B	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	Homo sapiens	7	ALA3886749	single protein format	BindingDB Database
16.	ALA3888529	B	Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).	Homo sapiens	7	ALA3886749	single protein format	BindingDB Database