# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3635884 | Binding | Inhibition of human recombinant GST-fused ALK5 expressed in Sf9 insect cells using casein as substrate by proprietary radioisotopic protein kinase assay | Homo sapiens | 15 | ALA3632597 | cell-based format | Scientific Literature | |
2. | ALA3635885 | Binding | Inhibition of human recombinant p38alpha expressed in Escherichia coli using ATF2 as substrate by proprietary radioisotopic protein kinase assay | Homo sapiens | 15 | ALA3632597 | assay format | Scientific Literature | |
3. | ALA3635886 | Binding | Selectivity index, ratio of IC50 for human recombinant p38alpha to IC50 for human recombinant ALK5 | Homo sapiens | 15 | ALA3632597 | assay format | Scientific Literature | |
4. | ALA3635887 | Binding | Inhibition of ALK5 in human HaCaT cells assessed as residual enzyme activity at 100 nM by p3TP-luciferase assay relative to control | Homo sapiens | 4 | ALA3632597 | cell-based format | Scientific Literature | |
5. | ALA4361670 | Binding | Inhibition of human recombinant GST-tagged ALK5 expressed in Sf9 insect cells using casein as substrate incubated for 60 mins in presence of [33P]-ATP by radiometric assay | Homo sapiens | 16 | ALA4359846 | cell-based format | Scientific Literature | |
6. | ALA4361671 | Binding | Inhibition of human recombinant untagged p38alpha expressed in Escherichia coli using ATF2 as substrate incubated for 60 mins in presence of [33P]-ATP by radiometric assay | Homo sapiens | 16 | ALA4359846 | assay format | Scientific Literature | |
7. | ALA4361672 | Binding | Selectivity index, ratio of IC50 for human recombinant untagged p38alpha expressed in Escherichia coli to IC50 for human recombinant GST-tagged ALK5 expressed in Sf9 insect cells | Homo sapiens | 16 | ALA4359846 | cell-based format | Scientific Literature | |
8. | ALA4361676 | Binding | Inhibition of ALK5 in human LX2 cells assessed as reduction in TGFbeta-induced collagen 1 production at 5 uM pretreated for 1 hr followed by TGFbeta addition and measured after 12 hrs by DAPI staining based immunocytochemistry analysis by confocal laser scanning microscopic method | Homo sapiens | 1 | ALA4359846 | cell-based format | Scientific Literature | |
9. | ALA4361677 | Binding | Inhibition of ALK5 in human LX2 cells assessed as reduction in TGFbeta-induced alphaSMA production at 5 uM pretreated for 1 hr followed by TGFbeta addition and measured after 12 hrs by DAPI staining based immunocytochemistry analysis by confocal laser scanning microscopic method | Homo sapiens | 1 | ALA4359846 | cell-based format | Scientific Literature | |
10. | ALA3269163 | Binding | Inhibition of ALK4 (unknown origin) by radioisotopic assay | Homo sapiens | 5 | ALA3259733 | single protein format | Scientific Literature | |
11. | ALA3270793 | Binding | Selectivity index, ratio of IC50 for ALK4 (unknown origin) to IC50 for ALK5 (unknown origin) | Homo sapiens | 4 | ALA3259733 | assay format | Scientific Literature | |
12. | ALA3270794 | Binding | Inhibition of RIPK2 (unknown origin) by radioisotopic assay | Homo sapiens | 4 | ALA3259733 | single protein format | Scientific Literature | |
13. | ALA3270795 | Binding | Selectivity index, ratio of IC50 for RIPK2 (unknown origin) to IC50 for ALK5 (unknown origin) | Homo sapiens | 4 | ALA3259733 | assay format | Scientific Literature | |
14. | ALA3270796 | Binding | Inhibition of VEGFR2 (unknown origin) by radioisotopic assay | Homo sapiens | 4 | ALA3259733 | single protein format | Scientific Literature | |
15. | ALA3270797 | Binding | Selectivity index, ratio of IC50 for VEGFR2 (unknown origin) to IC50 for ALK5 (unknown origin) | Homo sapiens | 4 | ALA3259733 | assay format | Scientific Literature | |
16. | ALA3270798 | Binding | Inhibition of ALK5 in human HaCaT cells assessed as inhibition of TGFbeta1-induced luciferase activity after 24 hrs by luciferase reporter gene assay | Homo sapiens | 4 | ALA3259733 | cell-based format | Scientific Literature | |
17. | ALA3270799 | Binding | Inhibition of ALK5 in mouse 4T1 cells assessed as inhibition of TGFbeta1-induced luciferase activity after 24 hrs by luciferase reporter gene assay | Mus musculus | 4 | ALA3259733 | cell-based format | Scientific Literature | |
18. | ALA3270806 | Functional | Antitumor activity against mouse B16 cells in mouse assessed as inhibition of tumor growth at 75 mg/kg, po bid relative to vehicle-treated control | Mus musculus | 1 | ALA3259733 | organism-based format | Scientific Literature | |
19. | ALA3270807 | Functional | Antitumor activity against mouse B16 cells in mouse assessed as suppression of tumor volume at 75 mg/kg, po bid relative to vehicle-treated control | Mus musculus | 1 | ALA3259733 | organism-based format | Scientific Literature | |
20. | ALA3270808 | Functional | Antitumor activity against mouse B16 cells in mouse assessed as inhibition of lymph node metastasis at 75 mg/kg, po bid relative to vehicle-treated control | Mus musculus | 1 | ALA3259733 | organism-based format | Scientific Literature |