# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2379327 | B | Inhibition of proteasome in human Jurkat cells assessed as accumulation of ubiquitinated protein at 50 uM by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
2. | ALA2379328 | B | Inhibition of trypsin-like activity of proteasome in human Jurkat cells at 50 uM after 30 mins by fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
3. | ALA2379329 | B | Inhibition of chymotrypsin-like activity of proteasome in human Jurkat cells assessed as residual activity at 50 uM after 30 mins by fluorescence assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA2379332 | B | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 100 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
5. | ALA2379333 | B | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 50 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
6. | ALA2379334 | B | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 25 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
7. | ALA2379335 | B | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 100 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
8. | ALA2379336 | B | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 50 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
9. | ALA2379507 | B | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 25 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
10. | ALA2379509 | F | Induction of apoptosis in human Jurkat cells assessed as dead cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 0.66%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
11. | ALA2379510 | F | Induction of apoptosis in human Jurkat cells assessed as late apoptotic cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 0.37%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
12. | ALA2379511 | F | Induction of apoptosis in human Jurkat cells assessed as early apoptotic cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 1.87%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
13. | ALA2379512 | F | Cell cycle arrest in human Jurkat cells assessed as accumulation at sub G0/G1 phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 4.81%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
14. | ALA2379513 | F | Cell cycle arrest in human Jurkat cells assessed as accumulation at G2/M phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 16.63%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
15. | ALA2379514 | F | Cell cycle arrest in human Jurkat cells assessed as accumulation at S phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 26.91%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
16. | ALA2379515 | F | Cell cycle arrest in human Jurkat cells assessed as accumulation at G0/G1 phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 52.59%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
17. | ALA2379516 | F | Induction of apoptosis in human Jurkat cells assessed as caspase 3/7 activation at 20 uM after overnight incubation by luminescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
18. | ALA2379517 | A | Cytotoxicity against human fibroblasts assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
19. | ALA2379553 | F | Cytotoxicity against human SW480 cells assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
20. | ALA2379554 | F | Cytotoxicity against human SKOV3 cells assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | cell-based format | Scientific Literature |