# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2379327 | Binding | Inhibition of proteasome in human Jurkat cells assessed as accumulation of ubiquitinated protein at 50 uM by Western blot analysis | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
2. | ALA2379328 | Binding | Inhibition of trypsin-like activity of proteasome in human Jurkat cells at 50 uM after 30 mins by fluorescence assay | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
3. | ALA2379329 | Binding | Inhibition of chymotrypsin-like activity of proteasome in human Jurkat cells assessed as residual activity at 50 uM after 30 mins by fluorescence assay relative to control | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
4. | ALA2379332 | Binding | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 100 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
5. | ALA2379333 | Binding | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 50 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
6. | ALA2379334 | Binding | Inhibition of trypsin-like activity of proteasome isolated from human Jurkat cells using Boc-LRR-AMC as substrate assessed as residual activity at 25 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
7. | ALA2379335 | Binding | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 100 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
8. | ALA2379336 | Binding | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 50 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
9. | ALA2379507 | Binding | Inhibition of chymotrypsin-like activity of proteasome isolated from human Jurkat cells using Suc-LLVY-AMC as substrate assessed as residual activity at 25 uM incubated for 30 mins followed by substrate addition measured for 2 hrs by fluorescence assay relative to control | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
10. | ALA2379509 | Functional | Induction of apoptosis in human Jurkat cells assessed as dead cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 0.66%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
11. | ALA2379510 | Functional | Induction of apoptosis in human Jurkat cells assessed as late apoptotic cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 0.37%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
12. | ALA2379511 | Functional | Induction of apoptosis in human Jurkat cells assessed as early apoptotic cells at 20 uM by propidium iodide/annexin V staining-based FACS flow cytometric analysis (Rvb = 1.87%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
13. | ALA2379512 | Functional | Cell cycle arrest in human Jurkat cells assessed as accumulation at sub G0/G1 phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 4.81%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
14. | ALA2379513 | Functional | Cell cycle arrest in human Jurkat cells assessed as accumulation at G2/M phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 16.63%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
15. | ALA2379514 | Functional | Cell cycle arrest in human Jurkat cells assessed as accumulation at S phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 26.91%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
16. | ALA2379515 | Functional | Cell cycle arrest in human Jurkat cells assessed as accumulation at G0/G1 phase at 20 uM by propidium iodide staining-based FACS flow cytometric analysis (Rvb = 52.59%) | Homo sapiens | 2 | ALA2375265 | cell-based format | Scientific Literature | |
17. | ALA2379516 | Functional | Induction of apoptosis in human Jurkat cells assessed as caspase 3/7 activation at 20 uM after overnight incubation by luminescence assay | Homo sapiens | 3 | ALA2375265 | cell-based format | Scientific Literature | |
18. | ALA2379517 | ADME | Cytotoxicity against human fibroblasts assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | ALA2375265 | cell-based format | Scientific Literature | |
19. | ALA2379553 | Functional | Cytotoxicity against human SW480 cells assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | ALA2375265 | cell-based format | Scientific Literature | |
20. | ALA2379554 | Functional | Cytotoxicity against human SKOV3 cells assessed as growth inhibition after 48 hrs by MTT assay | Homo sapiens | 11 | ALA2375265 | cell-based format | Scientific Literature |