#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2384327	B	Ratio of IWR1 EC50 to compound EC50 for tankyrase in human SW480 cells assessed as accumulation of axin2 after 24 hrs by Hoechst dye-based method	Homo sapiens	2		cell-based format	Scientific Literature
2.	ALA2384331	F	Unbound Cmax in po dosed CD-1 mouse to EC50 for tankyrase in human SW480 cells		3		organism-based format	Scientific Literature
3.	ALA2384332	B	Activation of rat PXR at 10 uM relative to control	Rattus norvegicus	3		single protein format	Scientific Literature
4.	ALA2384333	A	Inhibition of CYP3A4 (unknown origin)	Homo sapiens	3		single protein format	Scientific Literature
5.	ALA2384334	B	Inhibition of tankyrase in human SW480 cells assessed as accumulation of Axin2 after 24 hrs by Hoechst dye-based method in presence of FBA	Homo sapiens	3		cell-based format	Scientific Literature
6.	ALA2384339	A	AUCt in CD-1 mouse at 30 mg/kg, po	Mus musculus	16		organism-based format	Scientific Literature
7.	ALA2384340	A	Cmax in CD-1 mouse at 30 mg/kg, po	Mus musculus	16		organism-based format	Scientific Literature
8.	ALA2384341	A	Plasma protein binding in mouse	Mus musculus	8		cell-free format	Scientific Literature
9.	ALA2384345	P	Solubility of the compound in PBS at pH 7.4		8		small-molecule physicochemical format	Scientific Literature
10.	ALA2384346	P	Solubility of the compound in 0.01N HCl		8		small-molecule physicochemical format	Scientific Literature
11.	ALA2384347	P	Solubility of the compound in simulated intestinal fluid at pH 6.8		8		small-molecule physicochemical format	Scientific Literature
12.	ALA2384480	A	Intrinsic clearance in rat liver microsomes	Rattus norvegicus	30		microsome format	Scientific Literature
13.	ALA2384481	A	Intrinsic clearance in human liver microsomes	Homo sapiens	30		microsome format	Scientific Literature
14.	ALA2384482	B	Inhibition of tankyrase in human SW480 cells assessed as degradation of beta catenin after 40 to 48 hrs	Homo sapiens	31		cell-based format	Scientific Literature
15.	ALA2384483	B	Inhibition of tankyrase in human SW480 cells assessed as accumulation of axin2 after 24 hrs by Hoechst dye-based method	Homo sapiens	30		cell-based format	Scientific Literature
16.	ALA2384484	B	Inhibition of PARP2 (unknown origin) using histone as substrate after 1 hr by luminescence assay	Homo sapiens	5		single protein format	Scientific Literature
17.	ALA2384485	B	Inhibition of PARP1 (unknown origin) using histone as substrate after 1 hr by luminescence assay	Homo sapiens	5		single protein format	Scientific Literature
18.	ALA2384487	B	Inhibition of 6XHis-tagged tankyrase1 (1091 to 1325) (unknown origin) after 60 mins in presence of NAD	Homo sapiens	32		single protein format	Scientific Literature
19.	ALA3888461	B	Tankyrase Biochemical Assays: The tankyrase 1 biochemical activity of the compounds was assayed in the following assay buffer (50 mM MOPS pH7.5, 100 mM NaCl, 2.5 mM MgCl2, 0.01% Tween-20, 0.05% BSA, and 1 mM DTT) as follows: 0.25 nM of 6×HIS-tankyrase 1 (1091-1325) was incubated in the presence of compound (DMSO 1.85% final) in a Perkin Elmer 384 well Proxiplate Plus™ (cat. no. 6008289) with 400 nM of NAD for 60 minutes at room temperature. The assay was then stopped with the above assay buffer containing a 0.6 M inhibitor and the following detection components: 0.05 g/mL monoclonal anti-PAR antibody (Trevigen cat. no. 4335-MC-01K-AC) prebound for 60 minutes with 0.63 μg/mL protein G AlphaLisa® acceptor bead (Perkin Elmer cat. no. AL102M) and 5 μg/mL AlphaLisa® nickel chelate donor bead (Perkin Elmer cat. no. AS101M). The assay was incubated for 16 hours at room temperature in the dark and read on a Perkin Elmer Envision® multi label reader using the default program set with laser excitation at 680 nM and emission at 615 nM. The tankyrase 2 biochemical assay was performed using the procedure set forth above with respect to tankyrase 1 except that 4 nM 6×HIS-tankyrase 2 (946-1162) and 250 nM NAD were used.		175		single protein format	BindingDB Database