| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2406872 | B | Binding affinity to human recombinant Kelch-DC domain of Keap1 (321 to 609) expressed in HEK293T cells using [2H]dithiothreitol at 1 mM by NMR-HSQC analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 2. | ALA2406877 | B | Displacement of MR121-Nrf2 from human N-terminal His-tagged Kelch-DC domain of Keap1 (321 to 609) expressed in Escherichia coli BL21 (DE3) after 40 mins by 2D-FIDA assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 3. | ALA3620077 | B | Inhibition of Keap1 (unknown origin) interaction to Nrf2 | Homo sapiens | 8 | protein format | Scientific Literature | ||
| 4. | ALA4376060 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 5. | ALA4376062 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 6. | ALA4376065 | B | Binding affinity to Keap1 in mouse Hepa1c1c7 cells assessed as reduction in Keap1 interaction by measuring induction of NQO1 activity at 1 to 10 uM measured after 24 hrs | Mus musculus | 10 | cell-based format | Scientific Literature | ||
| 7. | ALA4376067 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 8. | ALA4376086 | B | Glutathione reactivity in HEPES buffer assessed as GSH-adduct formation at 500 uM measured after 10 to 30 mins by UHPLC-MS analysis | 5 | assay format | Scientific Literature | |||
| 9. | ALA4376087 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as compound-adduct formation by measuring molecular mass at 800 uM measured after 1 to 3 hrs by LC-MS analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 10. | ALA4376091 | U | Redox cycling activity of the compound assessed as H2O2 production measured after 15 mins in presence of TCEP by phenol red reagent based horseradish peroxidase coupled assay | 21 | assay format | Scientific Literature | |||
| 11. | ALA4376093 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins in presence of 0.01% Triton X-100 by fluorescence polarization assay | Homo sapiens | 13 | assay format | Scientific Literature | ||
| 12. | ALA4376101 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature by sypro orange dye based thermal shift assay | Homo sapiens | 11 | assay format | Scientific Literature |
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