| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2406872 | B | Binding affinity to human recombinant Kelch-DC domain of Keap1 (321 to 609) expressed in HEK293T cells using [2H]dithiothreitol at 1 mM by NMR-HSQC analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 2. | ALA2406873 | B | Activation of Nrf2 in human DLD1 cells assessed as increase of NQO1 protein level at 20 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 3. | ALA2406874 | B | Activation of Nrf2 in human DLD1 cells after 24 to 48 hrs by luciferase reporter gene assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA2406875 | B | Activation of Nrf2 in human DLD1 cells assessed as stabilization of Nrf2 protein at 20 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA2406876 | B | Binding affinity to human recombinant Kelch-DC domain of Keap1 (321 to 609) expressed in HEK293T cells assessed as covalent adduct formation with cysteines at 1 mM after 15 hrs by 2D-FIDA assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 6. | ALA2406877 | B | Displacement of MR121-Nrf2 from human N-terminal His-tagged Kelch-DC domain of Keap1 (321 to 609) expressed in Escherichia coli BL21 (DE3) after 40 mins by 2D-FIDA assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 7. | ALA3239748 | B | Binding affinity to kelch domain of Keap1 (unknown origin) assessed as change in melting temperature after 15 mins by differential scanning fluorimetry assay | Homo sapiens | 7 | single protein format | Scientific Literature | ||
| 8. | ALA3239781 | B | Activation of Nrf2 in rat PC12 cells assessed as increase of HO-1 mRNA level at 1 to 4 uM after 4.5 to 9 hrs by qRT-PCR analysis | Rattus norvegicus | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA3239782 | B | Activation of Nrf2 in rat PC12 cells assessed as increase of NQO-1 mRNA level at 1 to 4 uM after 4.5 to 9 hrs by qRT-PCR analysis | Rattus norvegicus | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA3242827 | B | Binding affinity to biotinylated Keap1 DC domain (unknown origin) by biolayer interferometry assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 11. | ALA3242829 | B | Displacement of FITC-LDEETGEFL-NH2 from Keap1 Kelch domain (unknown origin) after 30 mins by fluorescence polarization assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 12. | ALA3620077 | B | Inhibition of Keap1 (unknown origin) interaction to Nrf2 | Homo sapiens | 8 | protein format | Scientific Literature | ||
| 13. | ALA3637037 | B | Inhibition of Keap1-NRF2 interaction (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in FITC-9mer NRF2 peptide binding after 1 hr by fluorescence anisotropy assay | Homo sapiens | 33 | cell-based format | Scientific Literature | ||
| 14. | ALA3637038 | B | Binding affinity to the Keap1 (321 to 609 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by surface plasmon resonance assay | Homo sapiens | 25 | cell-based format | Scientific Literature | ||
| 15. | ALA3637040 | B | Activation of NRF2 in mouse MLE12 cells assessed as induction of HMOX1 expression at 10 uM incubated for 6 hrs by immunoblot assay | Mus musculus | 4 | cell-based format | Scientific Literature | ||
| 16. | ALA3637041 | B | Activation of NRF2 in mouse MLE12 cells assessed as increase in NRF2 level at 10 uM incubated for 6 hrs by immunoblot assay | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 17. | ALA3771930 | B | Inhibition of Nrf2:Keap1 interaction (unknown origin) assessed as induction of negative TdeltaS values by MR121 dye-labeled Nrf2-derived peptide based tracer displacement assay | Homo sapiens | 2 | protein format | Scientific Literature | ||
| 18. | ALA3771931 | B | Inhibition of Nrf2:Keap1 interaction (unknown origin) assessed as thermodynamic enthalpy changes by MR121 dye-labeled Nrf2-derived peptide based tracer displacement assay | Homo sapiens | 2 | protein format | Scientific Literature | ||
| 19. | ALA3870928 | B | Inhibition of recombinant GST-His-tagged Keap1-DC domain (321 to 609 residues) (unknown origin) expressed in Escherichia coli/FAM-labeled phosphorylated p62 (unknown origin) interaction measured after 30 mins by fluorescence polarization assay | Homo sapiens | 10 | assay format | Scientific Literature | ||
| 20. | ALA3870929 | B | Inhibition of recombinant GST-His-tagged Keap1-DC domain (321 to 609 residues) (unknown origin) expressed in Escherichia coli/FAM-labeled Nrf2 (unknown origin) interaction measured after 30 mins by fluorescence polarization assay | Homo sapiens | 10 | assay format | Scientific Literature |
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