# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2404522 | B | Activity at mouse SPHK1 transfected in mouse A20/2J cell lysates assessed as compound phosphorylation at 10 uM after 30 mins by autoradiography in presence of [gamma-32P]ATP | Mus musculus | 2 | cell-based format | Scientific Literature | ||
2. | ALA2404692 | B | Activity at mouse SPHK2 transfected in mouse A20/2J cell lysates assessed as compound phosphorylation at 10 uM after 30 mins by autoradiography in presence of [gamma-32P]ATP | Mus musculus | 2 | cell-based format | Scientific Literature | ||
3. | ALA2404703 | B | Inhibition of mouse SPHK1 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 2 to 4 uM incubated for 5 mins prior to substrate addition measured after 5 mins | Mus musculus | 2 | cell-based format | Scientific Literature | ||
4. | ALA2404704 | B | Inhibition of mouse SPHK2 in mouse A20/2J cells using [3-3H]sphingosine as substrate assessed as [3H]S1P formation at 2 to 4 uM incubated for 5 mins prior to substrate addition measured after 5 mins | Mus musculus | 2 | cell-based format | Scientific Literature |