Inhibition of human 11beta-HSD2 expressed in HEK293 cells assessed as [1,2,6,7-3H]-cortisol oxidation to [1,2-3H]-cortisone at 20 uM after 10 mins in presence of NAD+
Inhibition of human 11beta-HSD1 expressed in HEK293 cells assessed as [1,2-3H]-cortisone reduction to [1,2,6,7-3H]-cortisol after 10 mins in presence of NADPH
Inhibition of human 11beta-HSD1 expressed in HEK293 cells assessed as [1,2-3H]-cortisone reduction to [1,2,6,7-3H]-cortisol measured as residual activity at 20 uM after 10 mins relative to control in presence of NADPH