# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2434705 | B | Selectivity ratio for Escherichia coli TrmD over human SET7/9 | 10 | ALA2429843 | assay format | Scientific Literature | ||
2. | ALA2434709 | B | Selectivity ratio for Escherichia coli TrmD over human PRMT1 | 10 | ALA2429843 | assay format | Scientific Literature | ||
3. | ALA2434710 | B | Inhibition of human SET7/9 at 20 uM relative to control | Homo sapiens | 10 | ALA2429843 | single protein format | Scientific Literature | |
4. | ALA2434711 | B | Inhibition of human PRMT1 at 20 uM relative to control | Homo sapiens | 10 | ALA2429843 | single protein format | Scientific Literature | |
5. | ALA2434713 | B | Selectivity ratio of IC50 for Staphylococcus aureus TrmD to IC50 for Escherichia coli TrmD | 7 | ALA2429843 | assay format | Scientific Literature | ||
6. | ALA2434714 | B | Selectivity ratio of IC50 for Escherichia coli TrmD to IC50 for Pseudomonas aeruginosa TrmD | 7 | ALA2429843 | assay format | Scientific Literature | ||
7. | ALA2434715 | B | Inhibition of Pseudomonas aeruginosa TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Pseudomonas aeruginosa | 7 | ALA2429843 | assay format | Scientific Literature | |
8. | ALA2434716 | B | Inhibition of Klebsiella pneumoniae TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Klebsiella pneumoniae | 7 | ALA2429843 | organism-based format | Scientific Literature | |
9. | ALA2434717 | B | Inhibition of Acinetobacter baumannii TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Acinetobacter baumannii | 7 | ALA2429843 | assay format | Scientific Literature | |
10. | ALA2434718 | B | Inhibition of Haemophilus influenzae TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Haemophilus influenzae | 44 | ALA2429843 | assay format | Scientific Literature | |
11. | ALA2434719 | B | Inhibition of Staphylococcus aureus TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Staphylococcus aureus | 44 | ALA2429843 | assay format | Scientific Literature | |
12. | ALA2434720 | B | Inhibition of Escherichia coli TrmD using S-[methyl-3H] adenosyl-L-methionine as substrate assessed as leucine-tRNA methylation after 1 hr by scintillation proximity assay | Escherichia coli | 44 | ALA2429843 | assay format | Scientific Literature | |
13. | ALA4376261 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 1.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 26 | ALA4373727 | cell-based format | Scientific Literature | |
14. | ALA4376262 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 4.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
15. | ALA4376263 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 15 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
16. | ALA4376264 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
17. | ALA4376265 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Mycobacterium tuberculosis | 3 | ALA4373727 | assay format | Scientific Literature | |
18. | ALA4376266 | B | Binding affinity to full length Staphylococcus aureus tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay | Staphylococcus aureus | 3 | ALA4373727 | assay format | Scientific Literature | |
19. | ALA4376267 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as association rate constant by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
20. | ALA4376268 | B | Binding affinity to full length Mycobacterium tuberculosis tRNA (guanine(37)-N1)-methyltransferase expressed in Escherichia coli BL21 (DE3) assessed as association rate constant by surface plasmon resonance assay | Mycobacterium tuberculosis | 3 | ALA4373727 | assay format | Scientific Literature |