| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3706187 | Binding | BIOMOL Fluorescent-Based HDAC Assay: The BIOMOL fluorescent-based HDAC activity assay has been applied in In vitro HDACi assay for determination of IC50 values of the compounds of this invention. The assay has been carried out in white 96 well formats according to the BIOMOL's method. In order to optimize the experiment conditions and optinal, the exact concentration range of the Deacetylated Standard (biomol, KI-142) that will vary depending on the flurimeter model, the gain setting and the exact excitation and emission wavelengths used. We recommend diluting some of the standard to a relatively low concentration with Assay Buffer (an appropriate series of Deacetylated Standard dilutions from 20 uM to 0.3125 uM), and Assay Buffer as a time zero to a set of wells on the microtiter plate. Experimental to samples (five dilute groups: 100 uM, 20 uM, 4 uM, 0.8 uM, 0.16 uM) should be compared to a time zero. | Homo sapiens | 13 | ALA3638931 | single protein format | BindingDB Database | |
| 2. | ALA2443754 | Functional | Antiproliferative activity against human OVCAR5 cells after 48 hrs by sulforhodamine B assay | Homo sapiens | 7 | ALA2440014 | cell-based format | Scientific Literature | |
| 3. | ALA2443755 | Functional | Antiproliferative activity against human NCI-H460 cells after 48 hrs by sulforhodamine B assay | Homo sapiens | 7 | ALA2440014 | cell-based format | Scientific Literature | |
| 4. | ALA2443756 | Functional | Antiproliferative activity against human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay | Homo sapiens | 7 | ALA2440014 | cell-based format | Scientific Literature | |
| 5. | ALA2443757 | Functional | Antiproliferative activity against human MCF7 cells after 48 hrs by sulforhodamine B assay | Homo sapiens | 7 | ALA2440014 | cell-based format | Scientific Literature | |
| 6. | ALA2443758 | Functional | Antiproliferative activity against human HCT116 cells after 48 hrs by sulforhodamine B assay | Homo sapiens | 7 | ALA2440014 | cell-based format | Scientific Literature | |
| 7. | ALA2443759 | Binding | Inhibition of HDAC in human HeLa cell nuclear extracts using Fluor de Lys as substrate by fluorescence assay | Homo sapiens | 22 | ALA2440014 | protein format | Scientific Literature | |
| 8. | ALA2443760 | Binding | Inhibition of HDAC in human HeLa cell nuclear extracts using Fluor de Lys as substrate at 1 uM by fluorescence assay | Homo sapiens | 22 | ALA2440014 | protein format | Scientific Literature |
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