#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3089811	A	Metabolic stability in rat liver microsomes assessed as compound remaining after 10 mins	Rattus norvegicus	24		assay format	Scientific Literature
2.	ALA3089812	A	Metabolic stability in mouse liver microsomes assessed as compound remaining after 10 mins	Mus musculus	23		microsome format	Scientific Literature
3.	ALA3088369	A	Metabolic stability in human liver microsomes assessed as compound remaining after 10 mins	Homo sapiens	46		tissue-based format	Scientific Literature
4.	ALA3088370	B	Selectivity ratio of IC50 for KIT in human Mo7e cells to IC50 for FMS in mouse BMDM cells		47		cell-based format	Scientific Literature
5.	ALA3088371	B	Inhibition of KIT in human Mo7e cells assessed as inhibition of SCF-dependent cell proliferation	Homo sapiens	46		cell-based format	Scientific Literature
6.	ALA3088372	B	Inhibition of FMS in mouse BMDM cells	Mus musculus	57		cell-based format	Scientific Literature
7.	ALA3088374	B	Inhibition of FMS (unknown origin)	Homo sapiens	57		single protein format	Scientific Literature
8.	ALA3705109	B	Fluorescence Polarization Competition Assay: A fluorescence polarization competition immunoassay was used to measure compound inhibition of CSF-1R phosphorylation of tyrosine on a synthetic CSF-1R555-568 peptide (SYEGNSYTFIDPTQ). The assay was performed in black 96-well microplates (Cat #42-000-0117, Molecular Devices, Sunnyvale, Calif.). To each well, 5 μL of compound (in 4% DMSO) were mixed with 2 μL of 3.5 nM CSF-1R, 25 mM MgCl2 in assay buffer (100 mM HEPES (hydroxyethylpiperazineethylsodiumsulfonate), pH 7.5, 1 mM DTT (dithiothreitol), 0.01% Tween-20), and 2 μL of 1540 μM peptide in assay buffer. The kinase reaction was initiated by adding 1 μL of 10 mM ATP in assay buffer. The final concentrations in the 10 uL reaction mixture were 100 mM HEPES, pH 7.5, 1 mM DTT, 0.01% Tween-20, 2% DMSO, 308 μM SYEGNSYTFIDPTQ, 1 mM ATP, 5 mM MgCl2, and 0.7 nM CSF-1R.	Mus musculus	85		single protein format	BindingDB Database
9.	ALA3887764	B	Fluorescence Polarization Competition Immunoassay: A fluorescence polarization competition immunoassay was used to measure compound inhibition of CSF-1R phosphorylation of tyrosine on a synthetic CSF-1R555-568 peptide (SYEGNSYTFIDPTQ). The assay was performed in black 96-well microplates (Cat #42-000-0117, Molecular Devices, Sunnyvale, Calif.). To each well, 5 uL of compound (in 4% DMSO) were mixed with 2 uL of 3.5 nM CSF-1R, 25 mM MgCl2 in assay buffer (100 mM HEPES (hydroxyethylpiperazineethylsodiumsulfonate), pH 7.5, 1 mM DTT (dithiothreitol), 0.01% Tween-20), and 2 uL of 1540 uM peptide in assay buffer. The kinase reaction was initiated by adding 1 μL of 10 mM ATP in assay buffer. The final concentrations in the 10 uL reaction mixture were 100 mM HEPES, pH 7.5, 1 mM DTT, 0.01% Tween-20, 2% DMSO, 308 uM SYEGNSYTFIDPTQ, 1 mM ATP, 5 mM MgCl2, and 0.7 nM CSF-1R. Positive and negative control wells were included on each plate, where 4% DMSO in assay buffer was substituted for the compound; in addition, positive control wells received 1.2 uL of 50 mM EDTA (ethylenediaminetetraacetic acid) before the start of the reaction.		85		single protein format	BindingDB Database