# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3089724 | B | Inhibition of human ABHD12 overexpressed in HEK293 cells assessed as 2-AG hydrolysis at 1 uM by fluorescent glycerol assay | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
2. | ALA3089725 | B | Inhibition of human ABHD6 overexpressed in HEK293 cells assessed as 2-AG hydrolysis at 1 uM by fluorescent glycerol assay | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
3. | ALA3089786 | B | Inhibition of human recombinant MAGL overexpressed in HEK cells using 2-AG as substrate at 10 uM preincubated for 10 mins before substrate addition measured after 10 mins by HPLC analysis relative to control | Homo sapiens | 16 | cell-based format | Scientific Literature | ||
4. | ALA3088545 | B | Inhibition of human recombinant FAAH overexpressed in African green monkey COS7 cells using [3H]-anandamide as substrate at 10 uM preincubated for 10 mins before substrate addition measured after 10 mins by liquid scintillation counting analysis relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
5. | ALA3614381 | B | Inhibition of human FAAH expressed in COS7 cell membranes at 10 uM pre-incubated for 10 mins before [3H]-AEA substrate addition by liquid scintillation counting | Homo sapiens | 26 | cell-based format | Scientific Literature | ||
6. | ALA3614384 | B | Inhibition of human MAGL expressed in HEK293 cells at 10 uM pre-incubated for 10 mins before 2-AG substrate addition by HPLC method | Homo sapiens | 29 | cell-based format | Scientific Literature | ||
7. | ALA3614814 | B | Inhibition of human ABHD6 expressed in HEK293 cells assessed as remaining enzyme activity by measuring reduction in glycerol production from 1-AG hydrolysis at 1 uM pre-incubated for 30 mins before 1-AG substrate addition and measured 90 mins post substrate addition by fluorescence method | Homo sapiens | 31 | cell-based format | Scientific Literature |