| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3102002 | B | Inhibition of tankyrase in human DLD1 cells assessed as inhibition of Wnt gene transcription after 40 to 48 hrs by STF assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 2. | ALA3102003 | B | Inhibition of tankyrase in human SW480 cells assessed as degradation of total beta-catenin after 40 to 48 hrs | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 3. | ALA3102004 | B | Inhibition of tankyrase in human SW480 cells assessed as axin2 accumulation after 24 hrs by Hoechst dye-based assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 4. | ALA3102005 | B | Inhibition of PARP2 (unknown origin) | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 5. | ALA3102006 | B | Inhibition of PARP1 (unknown origin) | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 6. | ALA3102007 | B | Inhibition of recombinant TNKS2 (unknown origin) after 60 mins by spectrophotometric analysis | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 7. | ALA3102008 | B | Inhibition of recombinant 6XHis-tagged TNKS1 (1091 to 1325) (unknown origin) after 60 mins by spectrophotometric analysis | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 8. | ALA3888461 | B | Tankyrase Biochemical Assays: The tankyrase 1 biochemical activity of the compounds was assayed in the following assay buffer (50 mM MOPS pH7.5, 100 mM NaCl, 2.5 mM MgCl2, 0.01% Tween-20, 0.05% BSA, and 1 mM DTT) as follows: 0.25 nM of 6×HIS-tankyrase 1 (1091-1325) was incubated in the presence of compound (DMSO 1.85% final) in a Perkin Elmer 384 well Proxiplate Plus™ (cat. no. 6008289) with 400 nM of NAD for 60 minutes at room temperature. The assay was then stopped with the above assay buffer containing a 0.6 M inhibitor and the following detection components: 0.05 g/mL monoclonal anti-PAR antibody (Trevigen cat. no. 4335-MC-01K-AC) prebound for 60 minutes with 0.63 μg/mL protein G AlphaLisa® acceptor bead (Perkin Elmer cat. no. AL102M) and 5 μg/mL AlphaLisa® nickel chelate donor bead (Perkin Elmer cat. no. AS101M). The assay was incubated for 16 hours at room temperature in the dark and read on a Perkin Elmer Envision® multi label reader using the default program set with laser excitation at 680 nM and emission at 615 nM. The tankyrase 2 biochemical assay was performed using the procedure set forth above with respect to tankyrase 1 except that 4 nM 6×HIS-tankyrase 2 (946-1162) and 250 nM NAD were used. | 175 | single protein format | BindingDB Database |
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