#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3118127	B	Inhibition of human ALDH1B1 using propionaldehyde as substrate up to 100 uM preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	24		single protein format	Scientific Literature
2.	ALA3118128	B	Inhibition of human ALDH1A3 using propionaldehyde as substrate up to 100 uM preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	24		single protein format	Scientific Literature
3.	ALA3118129	B	Inhibition of human ALDH1A2 using propionaldehyde as substrate up to 100 uM preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	24		single protein format	Scientific Literature
4.	ALA3118131	B	Inhibition of human ALDH2 using propionaldehyde as substrate preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	24		single protein format	Scientific Literature
5.	ALA3118132	B	Inhibition of human ALDH1A1 using propionaldehyde as substrate preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	21		single protein format	Scientific Literature
6.	ALA3118331	B	Inhibition of human ALDH3A1-mediated benzaldehyde oxidation preincubated for 1 min followed by substrate addition by spectrophotometric analysis	Homo sapiens	24		single protein format	Scientific Literature
7.	ALA3887164	B	Inhibiton Assay: IC50 values were determined for CB29 and its analogs using propionaldehyde as the substrate for ALDH1A1 and ALDH2 or benzaldehyde as the substrate for ALDH3A1. The assays were performed on a Beckman DU-640 spectrophotometer at various concentrations of inhibitors ranging from 50 nM to 250 μM following a 1 minute pre-incubation. There was no pre-incubation time-dependence to the inhibition. All reactions were initiated by the addition of the aldehyde substrate. The inhibition curves were fit to the Logistic four parameter IC50 equation using the SigmaPlot (v11, StatSys). We characterized the mode of inhibition using steady-state kinetics through co-variation of inhibitor and substrate concentrations. The steady state kinetic measurements were performed in 100 mM Na2HPO4 buffer, pH 7.5. The reaction mixture contained 10 nM ALDH3A1, varied benzaldehyde (50 μM-800 μM; fixed NADP+, 1.5 mM) and varied inhibitor concentrations. In all cases−including the control reactions lacking inhibitors, the final reaction mixture contained 2% (v/v) DMSO. The reaction was initiated by addition of substrate and the initial rate of product formation was determined on a Beckman DU-640.	Homo sapiens	54		single protein format	BindingDB Database
8.	ALA4480788	F	Anti-trypanosomal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes after 72 hrs by Neubauer hemocytometric method	Trypanosoma cruzi	15		organism-based format	Scientific Literature
9.	ALA4480789	F	Anti-trypanosomal activity against Trypanosoma cruzi IRHOD/CO/2008/SN3 amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa-staining based microscopic analysis	Trypanosoma cruzi	15		organism-based format	Scientific Literature
10.	ALA4480790	A	Cytotoxicity against African green monkey Vero cells after 72 hrs by propidium iodide/fluorescein diacetate staining based flow cytometry	Chlorocebus sabaeus	15		cell-based format	Scientific Literature
11.	ALA4480791	T	Selectivity index, ratio of IC50 for African green monkey Vero cells to IC50 for Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes		15		assay format	Scientific Literature
12.	ALA4480792	T	Selectivity index, ratio of IC50 for African green monkey Vero cells to IC50 for Trypanosoma cruzi IRHOD/CO/2008/SN3 amastigotes infected in African green monkey Vero cells		15		assay format	Scientific Literature
13.	ALA4480795	B	Inhibition of PFK in Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes assessed as change in succinate excretion at IC25 after 96 hrs by 1H NMR analysis relative to control	Trypanosoma cruzi	5		assay format	Scientific Literature
14.	ALA4480796	B	Inhibition of PFK in Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes assessed as change in pyruvate excretion at IC25 after 96 hrs by 1H NMR analysis relative to control	Trypanosoma cruzi	5		assay format	Scientific Literature
15.	ALA4480797	B	Inhibition of PFK in Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes assessed as change in acetate excretion at IC25 after 96 hrs by 1H NMR analysis relative to control	Trypanosoma cruzi	5		assay format	Scientific Literature
16.	ALA4480798	B	Inhibition of PFK in Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes assessed as change in L-alanine excretion at IC25 after 96 hrs by 1H NMR analysis relative to control	Trypanosoma cruzi	5		assay format	Scientific Literature
17.	ALA4480799	B	Inhibition of PFK in Trypanosoma cruzi IRHOD/CO/2008/SN3 epimastigotes assessed as change in D-lactate excretion at IC25 after 96 hrs by 1H NMR analysis relative to control	Trypanosoma cruzi	5		assay format	Scientific Literature
18.	ALA4480800	B	Inhibition of Trypanosoma cruzi apoenzyme form of PFK	Trypanosoma cruzi	46		assay format	Scientific Literature