# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3124626 | B | Inhibition of TNFalpha-induced NFkappaB activation in rat INS-1E cells assessed as reduction of NFkappaB-DNA binding activity at 25 uM preincubated for 30 mins followed by cytokine challenge measured after 60 mins by electrophoretic mobility shift assay | Rattus norvegicus | 3 | ALA3120108 | cell-based format | Scientific Literature | |
2. | ALA3124627 | B | Inhibition of TNFalpha-induced NFkappaB activation in human MDA-MB-231 cells assessed as reduction of NFkappaB-DNA binding activity at 50 uM preincubated for 30 mins followed by cytokine challenge measured after 60 mins by electrophoretic mobility shift assay | Homo sapiens | 3 | ALA3120108 | cell-based format | Scientific Literature | |
3. | ALA3124629 | B | Inhibition of INFgamma-induced STAT1 activation in rat INS-1E cells assessed as reduction of STAT1-DNA binding activity at 25 uM preincubated for 30 mins followed by cytokine challenge measured after 60 mins by electrophoretic mobility shift assay | Rattus norvegicus | 3 | ALA3120108 | cell-based format | Scientific Literature | |
4. | ALA3124631 | B | Inhibition of INFgamma-induced STAT1 activation in human MDA-MD-231 cells assessed as reduction of STAT1-DNA binding activity at 50 uM preincubated for 30 mins followed by cytokine challenge measured after 60 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | ALA3120108 | cell-based format | Scientific Literature | |
5. | ALA3124633 | B | Inhibition of INFgamma-induced STAT1 activation in human MDA-MD-231 cells assessed as reduction of STAT1-DNA binding activity preincubated for 30 mins followed by cytokine challenge measured after 60 mins by electrophoretic mobility shift assay | Homo sapiens | 3 | ALA3120108 | cell-based format | Scientific Literature | |
6. | ALA3124634 | B | Binding affinity to STAT1 (unknown origin) by surface plasmon resonance assay | Homo sapiens | 4 | ALA3120108 | single protein format | Scientific Literature | |
7. | ALA4337973 | B | Inhibition of human topoisomerase 2alpha-mediated relaxation of supercoiled pSG483 DNA at 12.5 to 200 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 5 | ALA4334509 | single protein format | Scientific Literature | |
8. | ALA4337974 | B | Inhibition of human topoisomerase 2beta-mediated relaxation of supercoiled pSG483 DNA at 12.5 to 200 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 5 | ALA4334509 | single protein format | Scientific Literature | |
9. | ALA4337977 | B | Inhibition of human topoisomerase 2alpha-mediated DNA cleavage at 12.5 to 200 uM using supercoiled pSG483 as substrate after 30 mins by ethidium bromide staining based gel electrophoresis method | Homo sapiens | 4 | ALA4334509 | single protein format | Scientific Literature | |
10. | ALA4337978 | B | Inhibition of human topoisomerase 2beta-mediated DNA cleavage at 12.5 to 200 uM using supercoiled pSG483 as substrate after 30 mins by ethidium bromide staining based gel electrophoresis method | Homo sapiens | 4 | ALA4334509 | single protein format | Scientific Literature | |
11. | ALA4337982 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Vmax per mg protein at 10 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 136.7 +/- 14.7 mM/min) | Homo sapiens | 3 | ALA4334509 | single protein format | Scientific Literature | |
12. | ALA4337985 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Vmax per mg protein at 5 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 136.7 +/- 14.7 mM/min) | Homo sapiens | 2 | ALA4334509 | single protein format | Scientific Literature | |
13. | ALA4337986 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Vmax per mg protein at 20 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 136.7 +/- 14.7 mM/min) | Homo sapiens | 1 | ALA4334509 | single protein format | Scientific Literature | |
14. | ALA4337987 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Km at 10 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 0.4 +/- 0.1 mM) | Homo sapiens | 3 | ALA4334509 | single protein format | Scientific Literature | |
15. | ALA4337990 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Km at 5 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 0.4 +/- 0.1 mM) | Homo sapiens | 2 | ALA4334509 | single protein format | Scientific Literature | |
16. | ALA4337991 | B | Mixed-type inhibition of topoisomerase 2alpha ATPase activity (unknown origin) assessed as apparent Km at 20 uM using salmon sperm DNA as substrate measured every 30 secs for 90 mins in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay (Rvb = 0.4 +/- 0.1 mM) | Homo sapiens | 1 | ALA4334509 | single protein format | Scientific Literature |