# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3744488 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as decrease in TNF-alpha-induced GROalpha mRNA level pretreated with compound for 30 mins followed by addition of TNF-alpha measured after 12 hrs by RT-PCR analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
2. | ALA3744689 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as decrease in TNF-alpha-induced GROalpha mRNA level pretreated with compound for 30 mins followed by addition of TNF-alpha measured after 12 hrs by QR-PCR analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
3. | ALA3744690 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as decrease in TNF-alpha-induced GROalpha mRNA level at 10 uM pretreated for 30 mins followed by addition of TNF-alpha measured after 12 hrs by QR-PCR analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
4. | ALA3744691 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as inhibition of TNF-alpha-induced GROalpha promoter activation pretreated with compound for 30 mins followed by addition of TNF-alpha for 8 hrs by luciferase reporter gene assay | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
5. | ALA3744692 | Binding | Inhibition of TNF-alpha-induced IKK-alpha phosphorylation in human MDA-MB-231 cells at Ser176 residue at 5 and 10 uM pretreated for 30 mins followed by addition of TNF-alpha for 1 hr by immunoblot analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
6. | ALA3744693 | Binding | Inhibition of TNF-alpha-induced IKK-beta phosphorylation at Ser180 residue in human MDA-MB-231 cells at 5 and 10 uM pretreated for 30 mins followed by addition of TNF-alpha for 1 hr by immunoblot analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
7. | ALA3744694 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as inhibition of TNF-alpha-induced IkappaB phosphorylation at Ser-32 residue at 5 and 10 uM pretreated for 30 mins followed by addition of TNF-alpha for 1 hr by immunoblot analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
8. | ALA3744695 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as inhibition of TNF-alpha-induced p65/RelA phosphorylation at Ser-536 residue at 5 and 10 uM pretreated for 30 mins followed by addition of TNF-alpha for 1 hr by immunoblot analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
9. | ALA3744696 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as inhibition of TNF-alpha-induced p65/RelA phosphorylation at Ser-536 residue in nucleus at 10 uM pretreated for 30 mins followed by addition of TNF-alpha for 1 hr by immunofluorescence analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
10. | ALA3744697 | Binding | Inhibition of IKK in human MDA-MB-231 cells assessed as inhibition of TNF-alpha-induced NF-kappaB transcriptional activity pretreated with compound for 30 mins followed by addition of TNF-alpha for 8 hrs by luciferase reporter gene assay | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
11. | ALA3744698 | Functional | Inhibition of TNF-alpha-induced cell migration in human MDA-MB-231 cells at 10 uM pretreated for 30 mins followed by addition of TNF-alpha measured after 6 hrs by scratch wound healing assay | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
12. | ALA3744699 | Functional | Inhibition of TNF-alpha-induced cell motility in human MDA-MB-231 cells assessed as lamellipodium projection at 10 uM pretreated for 30 mins followed by addition of TNF-alpha measured after 10 mins by rhodamine- phalloidin staining based assay | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
13. | ALA3744700 | Functional | Inhibition of TNF-alpha-induced cell invasion in human MDA-MB-231 cells at 10 uM pretreated for 30 mins followed by addition of TNF-alpha measured after 24 hrs by matrigel invasion assay | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
14. | ALA3744701 | Functional | Inhibition of TNF-alpha-induced cell invasion in human MDA-MB-231 cells assessed as decrease in spindle-like protrusions at 10 uM pretreated for 30 mins followed by addition of TNF-alpha by microscopic analysis | Homo sapiens | 1 | ALA3739305 | cell-based format | Scientific Literature | |
15. | ALA3130132 | Functional | Growth inhibition of cisplatin-resistant human A2780cis cells after 7 days by crystal violet staining based clonogenic assay | Homo sapiens | 26 | ALA3124892 | cell-based format | Scientific Literature | |
16. | ALA4233942 | Functional | Induction of apoptosis in human A549 cells assessed as late apoptotic cells at 20 uM after 24 hrs by Annexn V-FITC/propidium iodide staining based flow cytometry (Rvb = 3%) | Homo sapiens | 1 | ALA4229450 | cell-based format | Scientific Literature | |
17. | ALA4233943 | Functional | Induction of apoptosis in human A549 cells assessed as necrotic cells at 20 uM after 24 hrs by Annexn V-FITC/propidium iodide staining based flow cytometry (Rvb = 10%) | Homo sapiens | 1 | ALA4229450 | cell-based format | Scientific Literature | |
18. | ALA4233944 | Functional | Anticlonogenic activity against human A549 cells at 20 uM after 7 days by crystal violet staining based assay | Homo sapiens | 1 | ALA4229450 | cell-based format | Scientific Literature | |
19. | ALA4233945 | Functional | Anticlonogenic activity against human NCI-H460 cells at 20 uM after 7 days by crystal violet staining based assay | Homo sapiens | 1 | ALA4229450 | cell-based format | Scientific Literature | |
20. | ALA4233946 | Functional | Anticlonogenic activity against human NCI-H1299 cells at 20 uM after 7 days by crystal violet staining based assay | Homo sapiens | 1 | ALA4229450 | cell-based format | Scientific Literature |