# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3705997 | Binding | Inhibition Assay: Thoroughly mixed test compounds, enzyme and reaction buffer, preincubated the mixture for 15 minutes at 37° C. and then primed reaction by adding substrate, successfully detecting fluorescence value at 460 nm for 5 minutes. At the same time, set the blank control group without substrate and solvent control group with DMSO replacing test compound, as well as positive control group of Vildagliptin (LAF-237) and Sitagliptin (MK-0431) [Bioorg. Med. Chem. Lett., 2005, 15, 4770-4773]. All final reaction volumes were 100 μL. Each concentration of each sample consisted of parallel wells in triplate. | Rattus norvegicus | 9 | ALA3638422 | single protein format | BindingDB Database | |
2. | ALA5217459 | Binding | Inhibition of DPP4 (unknown origin) using Gly-Pro-AMC as substrate incubated for 30 mins by fluorescence based enzyme immunoassay | Homo sapiens | 18 | ALA5214975 | single protein format | Scientific Literature | |
3. | ALA5217460 | Binding | Binding affinity to DPP4 (unknown origin) assessed as Km by Michaelis-Menten analysis | Homo sapiens | 18 | ALA5214975 | single protein format | Scientific Literature | |
4. | ALA5217466 | Binding | Inhibition of human DPP4 using Gly-Pro-AMC as substrate incubated for 30 mins by continuous fluorescent assay | Homo sapiens | 5 | ALA5214975 | single protein format | Scientific Literature |