PUBCHEM_BIOASSAY: qHTS for Inhibitors of binding or entry into cells for Lassa Virus. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID463114, AID540249]
PUBCHEM_BIOASSAY: Inhibitors of Eya2ED: Hit Confirmation in Primary Screen. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488837, AID488939]
PubChem BioAssay. A quantitative high throughput screen for small molecules that induce DNA re-replication in MCF 10a normal breast cells. (Class of assay: confirmatory)
Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins followed by OMFP substrate addition and further incubated for 30 mins by fluorescence based assay
Inhibition of GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli pre-incubated for 10 mins before phosphorylated H2AX peptide substrate addition and further incubated for 40 mins by malachite green-based secondary phosphatase assay
Inhibition of Flag-tagged Eya2 (unknown origin) expressed in human MCF7 cells co-transfected with pGL3-6xMEF3 assessed as reduction in Sixl-mediated transcription incubated fro 48 hrs by MEF3 DNA binding sites based luciferase reporter gene assay
Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein destabilization by measuring reduction in melting temperature in presence of 5 uM Mg2+ ions by thermal shift assay
Binding affinity to GST-fused human Eya2 ED (253 to 538 residues) expressed in Escherichia coli assessed as induction of protein stabilization by measuring melting temperature in presence of EDTA by thermal shift assay